Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
J Biomater Sci Polym Ed. 2021 Oct;32(15):1944-1965. doi: 10.1080/09205063.2021.1952380. Epub 2021 Oct 3.
In this study, hesperidin (HPN) loaded polyacrylonitrile (PAN)/polyethylene oxide (PEO) electrospun nanofibers were prepared for use as wound dressing. Accordingly, HPN loaded hybrid nanofibers were generated electrospinning. A full factorial design was then applied to evaluate the influence of formulation variables including PEO amount, HPN amount and total polymer amount on the nanofiber features. Fabricated membranes were evaluated in terms of morphology, diameter, entrapment efficiency (EE) %, drug loading (DL) %, release efficiency (RE) %, swelling % and mechanical properties. Analysis of the obtained data showed that the amount of PEO was the most effective factor impacting the swelling and release percentage; by raising the amount of PEO from 20% to 40%, the swelling % and release rate were considerably increased. The optimized nanofibers were found to be non-beaded, smooth and cylindrical with fiber diameter of 126.14 ± 23.96 nm, EE% of 38.58 ± 6.06, DL% of 5.36 ± 0.83, swelling % of 859.90 ± 33.49, RE % of 78.49 ± 0.21, UTS of 0.79 ± 0.13 MPa and Young's moduli of 20.91 ± 2.13 MPa. The physical state of HPN in optimized hybrid nanofibers was examined and the related XRD analysis revealed that HPN was either molecularly dispersed, or it existed in an amorphous state in the nanofibers. The studies also demonstrated that the wound healing rate in the case of HPN loaded nanofibers was higher when compared with other groups. Moreover, according to H&E and MT stain results, HPN loaded nanofibers did promote the regeneration of skin more effectively, as compared with HPN-free nanofibers. Overall, HPN loaded nanofibers mats prepared in this study have the potential to serve as wound dressings.
在这项研究中，制备了负载橙皮苷（HPN）的聚丙烯腈（PAN）/聚氧化乙烯（PEO）电纺纳米纤维，用作伤口敷料。因此，通过电纺生成负载 HPN 的混合纳米纤维。然后应用完全析因设计来评估包括 PEO 量、HPN 量和总聚合物量在内的配方变量对纳米纤维特性的影响。通过形态、直径、包封效率（EE）%、药物负载（DL）%、释放效率（RE）%、溶胀%和机械性能来评估制备的膜。对获得的数据进行分析表明，PEO 的量是影响溶胀和释放百分比的最有效因素；通过将 PEO 的量从 20%增加到 40%，溶胀%和释放速率显著增加。发现优化后的纳米纤维是非珠状的、光滑的和圆柱形的，纤维直径为 126.14 ± 23.96nm，EE%为 38.58 ± 6.06，DL%为 5.36 ± 0.83，溶胀%为 859.90 ± 33.49，RE%为 78.49 ± 0.21，UTS 为 0.79 ± 0.13MPa，杨氏模量为 20.91 ± 2.13MPa。研究还表明，与其他组相比，负载 HPN 的纳米纤维的伤口愈合速度更高。此外，根据 H&E 和 MT 染色结果，与无 HPN 的纳米纤维相比，负载 HPN 的纳米纤维更能有效地促进皮肤再生。总之，本研究中制备的负载 HPN 的纳米纤维垫具有作为伤口敷料的潜力。
