Kierstan M, Bucke C
Biotechnol Bioeng. 1977 Mar;19(3):387-97. doi: 10.1002/bit.260190309.
Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was deomonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days. Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels. Calcium alginate-immobilized chloroplasts were shown to perform the Hill reaction. Some experiments with immobilized mitochondria are reported.
将酿酒酵母细胞、马克斯克鲁维酵母细胞、菊粉酶、葡萄糖氧化酶、叶绿体和线粒体固定在海藻酸钙凝胶中。通过固定化的酿酒酵母制剂从葡萄糖溶液中生产乙醇的过程持续了总共23天，并且这种制剂的半衰期约为10天。使用固定化的马克斯克鲁维酵母、菊粉酶和葡萄糖氧化酶制剂来证明海藻酸钙凝胶的孔隙率和保留特性。结果表明，固定在海藻酸钙中的叶绿体能够进行希尔反应。还报道了一些关于固定化线粒体的实验。
