Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu Sichuan Province 611130, China.
J Immunol Res. 2020 Feb 11;2020:5948256. doi: 10.1155/2020/5948256. eCollection 2020.
Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after () infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological changes of the spleen caused by oxidative stress and inflammation in diet-induced obesity (DIO) mice challenged by . After infection, the spleen tissues were obtained in normal and DIO mice at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. Results revealed that DIO mice have higher contents of resistin, TNF-, IL-6, and IL-1 in the spleen than normal mice and lower concentrations of GSH-Px, SOD, and CAT and higher MDA than normal mice. After an intranasal drip of , the activities of GSH-Px, SOD, and CAT in the DIO mice were elevated and the content of MDA declined. The activities of SOD and CAT in the normal mice declined, and the content of MDA was elevated. Moreover, the contents of TNF-, IL-6, and IL-1 in the spleen declined in DIO mice at 24 and 72 h, although the contents of leptin, resistin, TNF-, IL-6, and IL-1 were elevated at 12 h. The contents of resistin, TNF-, IL-6, and IL-1 were elevated in normal mice at 12 and 24 h. Those results indicated that obesity elevated splenic oxidation and inflammatory levels, but it enhanced antioxidant capacity and reduced cytokine levels of the spleen in mice to resist splenic injury after an intranasal drip of .
肥胖小鼠在感染后支气管肺泡灌洗液中表现出更多的淋巴细胞和更轻微的肺损伤。然而，肥胖小鼠感染后脾脏是否对其产生影响仍不清楚。本研究旨在揭示氧化应激和炎症对饮食诱导肥胖（DIO）小鼠脾脏的组织病理学影响，以及 DIO 小鼠在感染后脾组织的变化。在感染后 0 h（未感染）、12 h、24 h 和 72 h，分别取正常和 DIO 小鼠的脾组织。结果表明，与正常小鼠相比，DIO 小鼠的脾组织中抵抗素、TNF-α、IL-6 和 IL-1 的含量更高，GSH-Px、SOD 和 CAT 的浓度更低，MDA 的含量更高。经鼻滴注后，DIO 小鼠的 GSH-Px、SOD 和 CAT 活性升高，MDA 含量降低。正常小鼠的 SOD 和 CAT 活性下降，MDA 含量升高。此外，DIO 小鼠在 24 和 72 h 时脾组织中 TNF-α、IL-6 和 IL-1 的含量下降，而在 12 h 时瘦素、抵抗素、TNF-α、IL-6 和 IL-1 的含量升高。正常小鼠在 12 和 24 h 时抵抗素、TNF-α、IL-6 和 IL-1 的含量升高。这些结果表明，肥胖会增加脾脏的氧化和炎症水平，但在经鼻滴注后，它会增强小鼠脾脏的抗氧化能力，降低细胞因子水平，从而抵抗脾脏损伤。
