Clough Mitchell, Chen Jerry L
Department of Biomedical Engineering, Boston University, Boston, USA.
Department of Biology, Boston University, Boston, USA.
Curr Opin Biomed Eng. 2019 Dec;12:95-101. doi: 10.1016/j.cobme.2019.11.004. Epub 2019 Nov 16.
While the action potential has long been understood to be the fundamental bit of information in brain, how these spikes encode representations of stimuli and drive behavior remains unclear. Large-scale neuronal recordings with cellular and spike-time resolution spanning multiple brain regions are needed to capture relevant network dynamics that can be sparse and distributed across the population. This review focuses on recent advancements in optical methods that have pushed the boundaries for simultaneous population recordings at increasing volumes, distances, depths, and speeds. The integration of these technologies will be critical for overcoming fundamental limits in the pursuit of whole brain imaging in mammalian species.
虽然长期以来人们一直认为动作电位是大脑中基本的信息单位，但这些脉冲如何编码刺激的表征并驱动行为仍不清楚。需要具有跨多个脑区的细胞和尖峰时间分辨率的大规模神经元记录，以捕捉可能稀疏且分布在群体中的相关网络动态。本综述聚焦于光学方法的最新进展，这些进展拓展了在更大体积、距离、深度和速度下进行同步群体记录的边界。这些技术的整合对于克服在哺乳动物全脑成像研究中的基本限制至关重要。
