Opt Lett. 2018 Apr 15;43(8):1914-1917. doi: 10.1364/OL.43.001914.
Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.
在神经科学领域，人们迫切需要易于采用、灵活且强大的容积成像工具，因为该领域需要具备以高时空分辨率对神经元及其网络进行成像的能力。使用轴向拉长的焦点近似贝塞尔光束，并结合双光子荧光显微镜，已经成功地实现了这一目标。在这里，我们展示了具有轴向扩展贝塞尔焦点的三光子荧光成像。我们使用基于轴棱锥的模块来产生具有不同数值孔径和轴向长度的贝塞尔焦点，并将这种体积成像工具应用于小鼠脑片的成像和小鼠大脑的活体成像。
