Huang Jiaqi, Wang Jianfei, Liu Shijie
Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, USA.
Mycology. 2023 Sep 13;15(4):523-537. doi: 10.1080/21501203.2023.2253827. eCollection 2024.
Lignocellulolytic enzymes play an important role in various industrial applications as well as the sustainable valorisation of lignocellulosic materials. Enzyme production using lignocellulosic fungi has shown great advantages such as high enzyme diversity, high production efficiency, and the availability of solid waste as raw materials. Agricultural waste, an abundant and non-food competitive feedstock, can be used to produce fungal lignocellulolytic enzymes. Pretreatment helps break down the complex structure of the raw material, thereby significantly improving product yield but also requiring more energy consumption. Multiple fermentation technologies, including submerged fermentation, solid-state fermentation, and co-culture, can be used for producing lignocellulolytic enzymes. Process optimisation may promote the yield and productivity of such enzymes without additional investment. Genetic engineering is also useful for enhancing enzyme production to meet industrial requirements. This review summarises the research progress in the fungal production of lignocellulolytic enzymes from various agricultural wastes via advanced fermentation strategies. It aims to provide technical references for the scale-up production of fungal lignocellulolytic enzymes.
木质纤维素酶在各种工业应用以及木质纤维素材料的可持续增值中发挥着重要作用。利用木质纤维素真菌生产酶已显示出诸多优势,如酶的多样性高、生产效率高以及可利用固体废物作为原料。农业废弃物是一种丰富且不与粮食竞争的原料,可用于生产真菌木质纤维素酶。预处理有助于分解原料的复杂结构,从而显著提高产品产量,但也需要更多的能源消耗。多种发酵技术,包括深层发酵、固态发酵和共培养,可用于生产木质纤维素酶。工艺优化可在不增加额外投资的情况下提高此类酶的产量和生产率。基因工程对于提高酶产量以满足工业需求也很有用。本综述总结了通过先进发酵策略利用各种农业废弃物真菌生产木质纤维素酶的研究进展。其目的是为真菌木质纤维素酶的规模化生产提供技术参考。