Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt; Chemistry Department, School of Pharmacy and Pharmaceutical Industries, Badr University in Cairo (BUC), Badr City, 11829, Cairo, Egypt.
Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt; Department of Chemistry, School of Sciences & Engineering, The American University in Cairo, New Cairo, Egypt.
Anal Chim Acta. 2020 Apr 1;1104:95-104. doi: 10.1016/j.aca.2020.01.002. Epub 2020 Jan 3.
Paper-based microfluidic device was designed with wax-printing to combine potentiometric, fluorimetric and colorimetric detection zones. This newly developed trimodal paper chip has been used for on-site determination of ketamine hydrochloride (KET) as a date rape drug in beverages. The device employed polyaniline nano-dispersion as conducting polymer in ion sensing paper electrodes designed to fit USB plug connector. Carbon dots-gold nanoparticles and cobalt thiocyanate were used in fluorescence and color detection zones, respectively. Cellular phone's camera facilitated the on-site fluorimetric and color detection. The implemented trimodal detection system exhibited specificity for KET detection in the presence of several other beverage interferences i.e., biogenic amines. This innovative sensor brings together analytical figures of merit for effective KET detection in single aliquot of spiked beverages. The proposed paper-based chip also fulfils WHO criteria for point-of-care devices posing the proposed trimodal paper device as an active part for rapid, on-site drug diagnostics and to be applied further for other similar drugs.
基于纸张的微流控装置采用蜡印技术将电势、荧光和比色检测区结合在一起。这种新开发的三模态纸芯片已用于现场测定盐酸氯胺酮（KET）作为饮料中的约会强奸药物。该设备采用聚苯胺纳米分散体作为离子传感纸电极中的导电聚合物，设计用于适配 USB 插头连接器。碳点-金纳米粒子和硫氰酸钴分别用于荧光和比色检测区。手机摄像头便于现场荧光和比色检测。所实现的三模态检测系统在存在几种其他饮料干扰物（即生物胺）的情况下表现出对 KET 检测的特异性。这种创新传感器汇集了用于有效检测单份加标饮料中 KET 的分析性能指标。所提出的基于纸张的芯片还符合世界卫生组织对即时检测设备的标准，使所提出的三模态纸张设备成为快速现场药物诊断的有效组成部分，并进一步应用于其他类似药物。
