Center of Molecular Metabolism, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094, China.
School of Pharmacy, Nanjing Medical University, Nanjing, 211166, China.
J Ethnopharmacol. 2020 May 23;254:112403. doi: 10.1016/j.jep.2019.112403. Epub 2020 Feb 25.
Gastrodiae Rhizoma (GR), a well-known and commonly-used TCM (Traditional Chinese Medicine) for treating headache, dizziness, tetanus, epilepsy, and etc., has been proven to relieve chronic atrophic gastritis (CAG). Due to its complex ingredients, the active fractions responsible for the treatment of CAG remain largely unknown.
To explore the underlying material and interpret its underlying mechanism, the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG was studied based on the H NMR metabolomics.
The rat model of CAG was established by autoimmune method. The modeled CAG rats were then treated with 4 polar parts (T1-4 in descending polarity, corresponding to water, n-butanol, ethyl acetate and petroleum ether extracts, respectively) of Gastrodiae Rhizoma for 21 consecutive days. The stomach and serum samples were collected and then subjected to histopathology observation, biochemical measurement (MDA, SOD, GSH, NO, XOD and pepsin), H NMR metabolic profiling and multivariate/univariate statistical analysis.
The results showed that T1 had the best therapeutic effect, T2 the second, and T3 and T4 the poorest with no obvious therapeutic effect, demonstrating that the effective components of Gastrodiae Rhizoma should be compounds of high polarity. T1 achieved good therapeutic effects due to the anti-inflammatory and anti-oxidant activities, and by rectifying the disturbed energy and amino acid metabolism in CAG model.
This integrated metabolomics approach proved the validity of the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG, providing new insights into the underlying mechanisms, and demonstrating the feasibility of metabolomics to evaluate efficacy of herbal drug, which is often difficult by traditional means.
天麻（GR）是一种著名且常用的中药，用于治疗头痛、头晕、破伤风、癫痫等疾病，已被证明可缓解慢性萎缩性胃炎（CAG）。由于其成分复杂，负责治疗 CAG 的活性成分在很大程度上仍不清楚。
基于 H NMR 代谢组学，研究天麻不同极性部位提取物对自身免疫性 CAG 的治疗作用，探讨其潜在的物质基础及其潜在机制。
采用自身免疫法建立 CAG 大鼠模型。然后，用天麻的 4 个极性部位（极性依次降低的 T1-4，分别对应水、正丁醇、乙酸乙酯和石油醚提取物）连续治疗 21 天。收集胃和血清样本，进行组织病理学观察、生化测量（MDA、SOD、GSH、NO、XOD 和胃蛋白酶）、H NMR 代谢组学分析和多变量/单变量统计分析。
结果表明，T1 的治疗效果最好，T2 次之，T3 和 T4 最差，没有明显的治疗效果，表明天麻的有效成分应该是高极性化合物。T1 之所以能取得良好的治疗效果，是因为其具有抗炎和抗氧化活性，同时纠正了 CAG 模型中紊乱的能量和氨基酸代谢。
本综合代谢组学方法证明了天麻不同极性部位提取物对自身免疫性 CAG 的治疗效果的有效性，为其潜在机制提供了新的见解，并证明了代谢组学评估草药药物疗效的可行性，这通常是传统方法难以实现的。
