Semmelweis University, Department of Pharmacognosy, Üllői u. 26, H-1085, Budapest, Hungary.
Semmelweis University, Department of Pharmacognosy, Üllői u. 26, H-1085, Budapest, Hungary.
J Pharm Biomed Anal. 2020 May 30;184:113184. doi: 10.1016/j.jpba.2020.113184. Epub 2020 Feb 19.
Human milk oligosaccharides (HMOs) are complex carbohydrates that exist naturally in mother's milk. Due to their myriad beneficial biological effects, HMOs are in the focus of current researches. Some of these complex carbohydrates are already commercially available utilizing their health-promoting benefits not just in infant formulas but in dietary supplements. Among the acidic oligosaccharides 3'- and 6'-sialyllactoses (SLs) are the most abundant in human milk. Extending the health benefits of these trisaccharides, 3'-SL and 6'-SL are expected to be active ingredients in infant- and follow-on formula and novel foods, therefore reliable and easy-to-perform analytical methods are urgently needed for their characterization. Herein we report a graphitized carbon liquid chromatography-mass spectrometry-based method for the separation and quantitation of sialyllactoses in human milk. Compounds were separated as their alditol forms and were quantified using maltotriose as internal standard. The developed bioanalytical method was validated in terms of selectivity, accuracy, precision, recovery, carry-over, matrix effects and stability. Two consecutive lactation periods of the same individual were monitored for the first time and a slight decrease in SL concentrations were found during the first month of the second lactation.
人乳寡糖（HMOs）是天然存在于母乳中的复杂碳水化合物。由于其众多有益的生物学效应，HMOs 是当前研究的焦点。其中一些复杂的碳水化合物已经在商业上利用其促进健康的益处，不仅在婴儿配方奶粉中，而且在膳食补充剂中。在酸性寡糖 3'- 和 6'-唾液酸乳糖（SLs）中，它们在人乳中最为丰富。为了扩展这些三糖的健康益处，3'-SL 和 6'-SL 有望成为婴儿配方奶粉和后续配方奶粉和新型食品的活性成分，因此迫切需要可靠且易于执行的分析方法来对其进行表征。在此，我们报告了一种基于石墨化碳液相色谱-质谱的方法，用于分离和定量人乳中的唾液酸乳糖。化合物以糖醇的形式分离，并使用麦芽三糖作为内标进行定量。该方法在选择性、准确性、精密度、回收率、交叉污染、基质效应和稳定性方面进行了验证。首次监测了同一个体的两个连续哺乳期，发现第二个哺乳期的第一个月 SL 浓度略有下降。
