Department of Environmental Health, Center for Public Health, Medical University of Vienna, A-1090 Vienna, Austria; Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna, A-1090 Vienna, Austria.
Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna, A-1090 Vienna, Austria; Environment Agency Austria, Spittelauer Lände 5, A-1090 Vienna, Austria.
Environ Int. 2020 Apr;137:105324. doi: 10.1016/j.envint.2019.105324. Epub 2020 Feb 25.
Perfluoroalkyl (PFAS) substances are widespread in the environment and in organisms. The fact that exposure to PFAS is associated with elevated cholesterol levels is a major concern for human health. Previous investigations, in which bovine serum albumin was frequently studied, indicate that PFOS, PFOA and PFNA bind to serum albumin. However, it is critical to know whether these and other PFAS have a preference for the protein or the lipid fraction in native human blood fractions. For this reason, blood samples from four young healthy volunteers (two women, two men, 23-31 years old) were used for protein size separation and fractionation by the Cohn method in combination with serial ultracentrifugation. The plasma fractions were analyzed for 11 PFAS using high-performance tandem mass spectrometry (HPLC-MS/MS). Although the data are based on a small sample, they clearly show that albumin is the most important carrier protein for PFOS, PFOA, PFHxS, PFNA and PFDA in native human plasma. These five compounds have very little or no affinity for lipoproteins. The confirmation of their transport through albumin is important for the epidemiology of PFAS. The present results must be verified by the examination of a larger number of persons.
全氟烷基（PFAS）物质广泛存在于环境和生物体内。接触 PFAS 与胆固醇水平升高有关，这是人类健康的一个主要关注点。以前的研究经常研究牛血清白蛋白，表明 PFOS、PFOA 和 PFNA 与血清白蛋白结合。然而，了解这些和其他 PFAS 是优先与天然人血液成分中的蛋白质还是脂质部分结合至关重要。出于这个原因，使用来自四名年轻健康志愿者（两名女性，两名男性，23-31 岁）的血液样本，通过 Cohn 方法结合连续超速离心进行蛋白质大小分离和分级。使用高效串联质谱法（HPLC-MS/MS）分析血浆部分中的 11 种 PFAS。尽管数据基于小样本，但它们清楚地表明，白蛋白是天然人血浆中 PFOS、PFOA、PFHxS、PFNA 和 PFDA 的最重要载体蛋白。这五种化合物与脂蛋白的亲和力很小或没有。通过白蛋白进行运输的确认对于 PFAS 的流行病学很重要。必须通过检查更多的人来验证这些结果。
