• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

具有聚集诱导发光活性的非共轭聚（乙烯基己内酰胺）作为用于细胞内温度成像的荧光温度计

AIE-active non-conjugated poly(-vinylcaprolactam) as a fluorescent thermometer for intracellular temperature imaging.

作者信息

Saha Biswajit, Ruidas Bhuban, Mete Sourav, Mukhopadhyay Chitrangada Das, Bauri Kamal, De Priyadarsi

机构信息

Polymer Research Centre and Centre for Advanced Functional Materials , Department of Chemical Sciences , Indian Institute of Science Education and Research Kolkata , Nadia , Mohanpur - 741246 , West Bengal , India . Email:

Centre for Healthcare Science and Technology , Indian Institute of Engineering Science and Technology , Shibpur, P.O. Botanic Garden , Howrah , West Bengal 711103 , India . Email:

出版信息

Chem Sci. 2019 Oct 28;11(1):141-147. doi: 10.1039/c9sc04338a. eCollection 2020 Jan 7.

DOI:10.1039/c9sc04338a
PMID:32110365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7012023/
Abstract

Since temperature is one of the most significant physiological parameters that dictate the cellular status of living organisms, accurate intracellular temperature measurement is crucial and a valuable biomarker for the diagnosis and treatment of diseases. Herein, we introduce the foremost example of a non-conjugated polymer as a next generation fluorescent thermometer which is capable of addressing the key shortcomings including toxicity and thermal-induced fluorescence quenching associated with π-π conjugated system-based thermometers developed so far. We revealed, for the first time, the unique photophysical and aggregation-induced emission (AIE) characteristics of well-known thermoresponsive poly(-vinylcaprolactam) (PNVCL) devoid of any classical fluorophore entity. PNVCL underwent a coil to globular conformational transition in an aqueous medium and appeared to be fluorescent above its lower critical solution temperature (LCST) near body temperature (38 °C). Eventually, this intriguing aspect enabled higher cellular uptake of PNVCL at the LCST boundary. By virtue of the AIE effect, the thermo-induced aggregation phenomenon has been ingeniously utilized to apply PNVCL as a novel fluorescent thermometer for intracellular temperature determination.

摘要

由于温度是决定生物体细胞状态的最重要生理参数之一，准确测量细胞内温度至关重要，是疾病诊断和治疗的重要生物标志物。在此，我们介绍了一种非共轭聚合物作为下一代荧光温度计的首要示例，它能够解决包括毒性和热致荧光猝灭等关键缺点，这些缺点与迄今为止开发的基于π-π共轭体系的温度计相关。我们首次揭示了著名的热响应性聚（乙烯基己内酰胺）（PNVCL）独特的光物理和聚集诱导发光（AIE）特性，该聚合物不含任何经典荧光团实体。PNVCL在水性介质中经历了从线圈到球状的构象转变，并且在接近体温（38°C）的下临界溶液温度（LCST）以上似乎具有荧光性。最终，这一有趣的方面使得PNVCL在LCST边界处具有更高的细胞摄取率。借助AIE效应，热诱导聚集现象已被巧妙地用于将PNVCL用作测定细胞内温度的新型荧光温度计。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/a3ec24de79ae/c9sc04338a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/8cfcfe978297/c9sc04338a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/b6ff91e85f57/c9sc04338a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/3a97d894fa33/c9sc04338a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/999701959992/c9sc04338a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/a3ec24de79ae/c9sc04338a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/8cfcfe978297/c9sc04338a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/b6ff91e85f57/c9sc04338a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/3a97d894fa33/c9sc04338a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/999701959992/c9sc04338a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/92d7/7012023/a3ec24de79ae/c9sc04338a-f4.jpg

相似文献

1
AIE-active non-conjugated poly(-vinylcaprolactam) as a fluorescent thermometer for intracellular temperature imaging.具有聚集诱导发光活性的非共轭聚（乙烯基己内酰胺）作为用于细胞内温度成像的荧光温度计
Chem Sci. 2019 Oct 28;11(1):141-147. doi: 10.1039/c9sc04338a. eCollection 2020 Jan 7.
2
Synthesis, characterisation and phase transition behaviour of temperature-responsive physically crosslinked poly (N-vinylcaprolactam) based polymers for biomedical applications.用于生物医学应用的温敏型物理交联聚（N-乙烯基己内酰胺）基聚合物的合成、表征及相转变行为。
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:130-139. doi: 10.1016/j.msec.2017.03.241. Epub 2017 Mar 29.
3
Biological Stimuli-Induced Phase Transition of a Synthesized Block Copolymer: Preferential Interactions between PNIPAM--PNVCL and Heme Proteins.生物刺激诱导的合成嵌段共聚物的相变：聚N-异丙基丙烯酰胺-聚N-乙烯基己内酰胺与血红素蛋白之间的优先相互作用
Langmuir. 2021 Feb 9;37(5):1682-1696. doi: 10.1021/acs.langmuir.0c02900. Epub 2021 Jan 25.
4
Characterization of Thermoresponsive Poly-N-Vinylcaprolactam Polymers for Biological Applications.用于生物应用的热响应性聚-N-乙烯基己内酰胺聚合物的表征
Polymers (Basel). 2021 Aug 8;13(16):2639. doi: 10.3390/polym13162639.
5
Multifaceted thermoresponsive poly(N-vinylcaprolactam) coupled with carbon dots for biomedical applications.用于生物医学应用的多面热响应性聚（N-乙烯基己内酰胺）与碳点耦合
Mater Sci Eng C Mater Biol Appl. 2016 Apr 1;61:492-8. doi: 10.1016/j.msec.2015.12.070. Epub 2015 Dec 31.
6
Atomistic molecular dynamics simulations of the LCST conformational transition in poly(N-vinylcaprolactam) in water.聚（N-乙烯基己内酰胺）在水中的最低临界溶液温度构象转变的原子分子动力学模拟
J Mol Graph Model. 2019 Jul;90:51-58. doi: 10.1016/j.jmgm.2019.04.004. Epub 2019 Apr 12.
7
Preparation of a Mini-Library of Thermo-Responsive Star (NVCL/NVP-VAc) Polymers with Tailored Properties Using a Hexafunctional Xanthate RAFT Agent.使用六官能团黄原酸酯RAFT试剂制备具有定制性能的热响应性星形（NVCL/NVP-VAc）聚合物微型文库。
Polymers (Basel). 2017 Dec 24;10(1):20. doi: 10.3390/polym10010020.
8
A simple yet effective AIE-based fluorescent nano-thermometer for temperature mapping in living cells using fluorescence lifetime imaging microscopy.基于聚集诱导发光的荧光纳米温度计用于利用荧光寿命成像显微镜在活细胞中进行温度成像：一种简单而有效的方法。
Nanoscale Horiz. 2020 Mar 2;5(3):488-494. doi: 10.1039/c9nh00693a.
9
Influence of the Near Molecular Vicinity on the Temperature Regulated Fluorescence Response of Poly(N-vinylcaprolactam).近分子邻域对聚（N-乙烯基己内酰胺）温度调节荧光响应的影响
Polymers (Basel). 2016 Mar 25;8(4):109. doi: 10.3390/polym8040109.
10
Initiated chemical vapor deposition of thermoresponsive poly(N-vinylcaprolactam) thin films for cell sheet engineering.用于细胞片层工程的温敏性聚（N-乙烯基己内酰胺）薄膜的起始化学气相沉积。
Acta Biomater. 2013 Aug;9(8):7691-8. doi: 10.1016/j.actbio.2013.04.049. Epub 2013 May 7.

引用本文的文献

1
Smart aggregation-induced emission polymers: Preparation, properties and bio-applications.智能聚集诱导发光聚合物：制备、性质及生物应用
Smart Mol. 2023 Mar 27;1(1):e20220008. doi: 10.1002/smo.20220008. eCollection 2023 Jun.
2
Synthesis and Characterization of Multilayer 3D Chiral Polymers with Enhanced Optical Properties.具有增强光学性质的多层三维手性聚合物的合成与表征
Molecules. 2025 Mar 31;30(7):1567. doi: 10.3390/molecules30071567.
3
The Bioimaging Story of AIEgens.聚集诱导发光（AIE）gens的生物成像故事。

本文引用的文献

1
Temperature-Responsive Fluorescence Polymer Probes with Accurate Thermally Controlled Cellular Uptakes.具有精确热控细胞摄取功能的温度响应型荧光聚合物探针。
ACS Macro Lett. 2014 Mar 18;3(3):281-285. doi: 10.1021/mz5000569. Epub 2014 Mar 5.
2
Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy.使用阳离子线性荧光聚合物温度计和荧光寿命成像显微镜进行温度成像。
Nat Protoc. 2019 Apr;14(4):1293-1321. doi: 10.1038/s41596-019-0145-7. Epub 2019 Mar 22.
3
An enzyme-activatable probe liberating AIEgens: on-site sensing and long-term tracking of β-galactosidase in ovarian cancer cells.
Chem Biomed Imaging. 2023 Jul 3;1(6):509-521. doi: 10.1021/cbmi.3c00056. eCollection 2023 Sep 25.
4
Recent Progress in Nonconventional Luminescent Macromolecules and their Applications.非传统发光大分子及其应用的最新进展
Macromolecules. 2024 Jun 11;57(12):5561-5577. doi: 10.1021/acs.macromol.4c00186. eCollection 2024 Jun 25.
5
Luminescence Thermometry with Nanoparticles: A Review.纳米颗粒发光测温法综述
Nanomaterials (Basel). 2023 Nov 5;13(21):2904. doi: 10.3390/nano13212904.
6
A Self-Assemble Supramolecular Film with Humidity Visualization Enabled by Clusteroluminescence.一种具有团簇发光实现湿度可视化功能的自组装超分子薄膜。
Adv Sci (Weinh). 2024 Jan;11(1):e2304946. doi: 10.1002/advs.202304946. Epub 2023 Nov 9.
7
AIEgen orthopalladated hybrid polymers for efficient inactivation of the total coliforms in urban wastewater.用于高效灭活城市废水中总大肠菌群的 AIEgen 正多钯杂化聚合物。
Sci Rep. 2023 Sep 22;13(1):15790. doi: 10.1038/s41598-023-41315-x.
8
Designing Nonconventional Luminescent Materials with Efficient Emission in Dilute Solutions via Modulation of Dynamic Hydrogen Bonds.通过调制动态氢键设计在稀溶液中具有高效发射的非常规发光材料。
Molecules. 2023 Jul 6;28(13):5240. doi: 10.3390/molecules28135240.
9
Programmed Macromolecular Assembly by Dipole-Dipole Interactions with Aggregation-Induced Enhanced Emission in Aqueous Medium.通过偶极-偶极相互作用在水介质中进行的程序化大分子组装及聚集诱导增强发光
ACS Polym Au. 2022 Jan 20;2(4):223-231. doi: 10.1021/acspolymersau.1c00054. eCollection 2022 Aug 10.
10
Thermal Probing Techniques for a Single Live Cell.用于单个活细胞的热探测技术。
Sensors (Basel). 2022 Jul 7;22(14):5093. doi: 10.3390/s22145093.
一种可激活酶释放聚集诱导发光分子的探针：卵巢癌细胞中β-半乳糖苷酶的原位传感与长期追踪
Chem Sci. 2018 Oct 9;10(2):398-405. doi: 10.1039/c8sc04266g. eCollection 2019 Jan 14.
4
Fluorescence of Nonaromatic Organic Systems and Room Temperature Phosphorescence of Organic Luminogens: The Intrinsic Principle and Recent Progress.非芳香有机体系的荧光和有机发光体的室温磷光：内在原理和最新进展。
Small. 2018 Sep;14(38):e1801560. doi: 10.1002/smll.201801560. Epub 2018 Aug 2.
5
Temperature regulates NF-κB dynamics and function through timing of A20 transcription.温度通过 A20 转录的时间来调节 NF-κB 的动态和功能。
Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5243-E5249. doi: 10.1073/pnas.1803609115. Epub 2018 May 14.
6
Clustering-Triggered Emission and Persistent Room Temperature Phosphorescence of Sodium Alginate.藻酸钠的聚集触发发射和室温磷光持续。
Biomacromolecules. 2018 Jun 11;19(6):2014-2022. doi: 10.1021/acs.biomac.8b00123. Epub 2018 Mar 30.
7
Progress and Trends in AIE-Based Bioprobes: A Brief Overview.基于聚集诱导发光的生物探针的研究进展与趋势：简要综述。
ACS Appl Mater Interfaces. 2018 Apr 18;10(15):12217-12261. doi: 10.1021/acsami.7b14343. Epub 2017 Nov 28.
8
Intracellular temperature measurements with fluorescent polymeric thermometers.使用荧光聚合物温度计进行细胞内温度测量。
Chem Commun (Camb). 2017 Oct 5;53(80):10976-10992. doi: 10.1039/c7cc06203f.
9
Aggregation-induced emission: mechanistic study of the clusteroluminescence of tetrathienylethene.聚集诱导发光：四噻吩乙烯簇发光的机理研究
Chem Sci. 2017 Apr 1;8(4):2629-2639. doi: 10.1039/c6sc05192h. Epub 2017 Jan 11.
10
Clustering-Triggered Emission of Nonconjugated Polyacrylonitrile.无规共聚聚丙烯腈的聚集触发发光。
Small. 2016 Dec;12(47):6586-6592. doi: 10.1002/smll.201601545. Epub 2016 Sep 8.