用于单个活细胞的热探测技术。

School of Microelectronics, Shandong University, Jinan 250100, China.

School of Electronics, Peking University, Beijing 100871, China.

Sensors (Basel). 2022 Jul 7;22(14):5093. doi: 10.3390/s22145093.

Temperature is a significant factor in determining and characterizing cellular metabolism and other biochemical activities. In this study, we provide a brief overview of two important technologies used to monitor the local temperatures of individual living cells: fluorescence nano-thermometry and an array of micro-/nano-sized thin-film thermocouples. We explain some key technical issues that must be addressed and optimised for further practical applications, such as in cell biology, drug selection, and novel antitumor therapy. We also offer a method for combining them into a hybrid measuring system.

温度是决定和描述细胞代谢和其他生化活动的重要因素。在这项研究中，我们简要介绍了两种用于监测单个活细胞局部温度的重要技术：荧光纳米测温法和微/纳米尺寸薄膜热电偶阵列。我们解释了一些必须解决的关键技术问题，并针对进一步的实际应用进行了优化，例如在细胞生物学、药物筛选和新型抗肿瘤治疗中。我们还提供了一种将它们组合成混合测量系统的方法。