Pham Van Hieu, Kan Liugang, Huang Jinyu, Geng Yanqiang, Zhen Wenrui, Guo Yuming, Abbas Waseem, Wang Zhong
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.
2Faculty of Animal Science and Veterinary Medicine, Thai Nguyen University Agriculture and Forestry, Thai Nguyen, Vietnam.
J Anim Sci Biotechnol. 2020 Feb 21;11:18. doi: 10.1186/s40104-019-0421-y. eCollection 2020.
The poultry industry is in need of effective antibiotic alternatives to control outbreaks of necrotic enteritis (NE) due to . In the present study, we investigated the effects of dietary supplementation with a blend of encapsulated essential oils and organic acids (BLJ) on growth performance and gut health using a coinfection model of NE in broiler chickens.
Two hundred and eighty-eight one-day-old male Arbor Acres broiler chicks were randomly assigned using a 2 × 2 factorial design into two groups fed either 0 or 500 mg/kg dietary BLJ and co-challenged (or not challenged for the control) with spp./.
Infected birds fed the BLJ-supplemented diet exhibited an improved feed conversion ratio throughout the trial ( < 0.01), a higher villus height and villus height/crypt depth ratio, and reduced intestinal counts, liver carriage, gut lesion scores and serum fluorescein isothiocyanate dextran (FITC-D) concentrations at 7 d post-infection compared with those of birds without BLJ supplementation ( < 0.05). NE-infected birds fed BLJ exhibited significantly upregulated claudin-1 and mRNA levels ( < 0.05), increased mRNA expression and significantly downregulated , and mRNA levels in the jejunum at 7 d post-infection compared with those in birds without BLJ supplementation ( < 0.05). Compared with the uninfected and untreated birds, the uninfected birds fed BLJ displayed increased relative abundances of and but reduced Rikenellaceae levels. Compared with the unsupplemented NE-challenged birds, infected birds fed BLJ showed an increased relative abundance of Unclassified_Lachnospiraceae and a significantly decreased relative abundance of Erysipelotrichaceae.
BLJ supplementation improved growth performance and gut health in NE-infected broiler chickens by strengthening the intestinal barrier function, positively modulating the gut microbiota community and differentially regulating intestinal immune responses. Our results also suggested that adding BLJ effectively controlled NE infections after experimental and coinfection.
家禽业需要有效的抗生素替代品来控制由[未提及具体病因]引起的坏死性肠炎（NE）疫情。在本研究中，我们使用肉鸡坏死性肠炎共感染模型，研究了日粮中添加包封精油和有机酸混合物（BLJ）对生长性能和肠道健康的影响。
288只1日龄雄性艾维茵肉鸡雏鸡，采用2×2析因设计随机分为两组，分别饲喂0或500mg/kg日粮BLJ，并与[未提及具体菌种]共同攻毒（对照组不攻毒）。
在整个试验期间，饲喂添加BLJ日粮的感染鸡的饲料转化率有所提高（P<0.01），绒毛高度和绒毛高度/隐窝深度比值更高，与未添加BLJ的鸡相比，感染后7天肠道[未提及具体细菌名称]数量、肝脏[未提及具体细菌名称]携带量、肠道病变评分和血清异硫氰酸荧光素葡聚糖（FITC-D）浓度降低（P<0.05）。与未添加BLJ的鸡相比，感染NE并饲喂BLJ的鸡在感染后7天空肠中claudin-1和[未提及具体基因名称]mRNA水平显著上调（P<0.05），[未提及具体基因名称]mRNA表达增加，[未提及具体基因名称]、[未提及具体基因名称]和[未提及具体基因名称]mRNA水平显著下调（P<0.05）。与未感染且未处理的鸡相比，饲喂BLJ的未感染鸡[未提及具体细菌属名]和[未提及具体细菌属名]的相对丰度增加，但理研菌科水平降低。与未添加BLJ的NE攻毒鸡相比，感染并饲喂BLJ的鸡未分类毛螺菌科的相对丰度增加，丹毒丝菌科的相对丰度显著降低。
添加BLJ通过增强肠道屏障功能、积极调节肠道微生物群落和差异调节肠道免疫反应，改善了感染NE的肉鸡的生长性能和肠道健康。我们的结果还表明，在实验性[未提及具体细菌名称]和[未提及具体细菌名称]共感染后，添加BLJ可有效控制NE感染。
