Department of Urology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, 518020, China.
BMC Microbiol. 2020 Feb 28;20(1):41. doi: 10.1186/s12866-020-01734-6.
The dogma that urine is sterile in healthy individuals has been overturned by recent studies applying molecular-based methods. Mounting evidences indicate that dysbiosis of the urinary microbiota is associated with several urological diseases. In this study, we aimed to investigate the urinary microbiome of male patients with calcium-based kidney stones and compare it with those of healthy individuals.
The diversity of the urinary microbiota in kidney stone patients was significantly lower than that of healthy controls based on the Shannon and Simpson index (P < 0.001 for both indices). The urinary microbiota structure also significantly differed between kidney stone patients and healthy controls (ANOSIM, R = 0.11, P < 0.001). Differential representation of inflammation associated bacteria (e.g., Acinetobacter) and several enriched functional pathways were identified in the urine of kidney stones patients. Meanwhile, we found the species diversity, overall composition of microbiota and predicted functional pathways were similar between bladder urine and renal pelvis urine in kidney stone patients.
A marked dysbiosis of urinary microbiota in male patients with calcium-based kidney stones was observed, which may be helpful to interpret the association between bacteria and calcium-based kidney stones.
最近应用基于分子的方法的研究推翻了健康个体尿液无菌的教条。越来越多的证据表明，尿微生物群失调与几种泌尿科疾病有关。在这项研究中，我们旨在研究男性钙基肾结石患者的尿微生物组，并将其与健康个体进行比较。
基于 Shannon 和 Simpson 指数，肾结石患者的尿微生物多样性明显低于健康对照组（两个指数的 P 值均<0.001）。肾结石患者和健康对照组的尿微生物群结构也有显著差异（ANOSIM，R=0.11，P<0.001）。在肾结石患者的尿液中发现了与炎症相关的细菌（例如，不动杆菌）和几个富集的功能途径的差异表达。同时，我们发现肾结石患者的膀胱尿和肾盂尿的物种多样性、微生物群的整体组成和预测功能途径相似。
观察到男性钙基肾结石患者的尿微生物群明显失调，这可能有助于解释细菌与钙基肾结石之间的关系。
