桑椹多糖的结构表征、修饰及保肝作用研究。
Structural characterization, modification and hepatoprotective effects of polysaccharide from Mori Fructus.
机构信息
Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou 550001, PR China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou 550001, PR China.
Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou 550001, PR China; Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guizhou Normal University, Guiyang, Guizhou 550001, PR China; The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, Guizhou 550001, PR China.
出版信息
Int J Biol Macromol. 2020 Jun 15;153:357-363. doi: 10.1016/j.ijbiomac.2020.02.300. Epub 2020 Feb 26.
In this paper, three purified Mori Fructus (M. Fructus) polysaccharides (MFP-1, MFP-2 and MFP-3) were successfully obtained from M. Fructus. Structural modification methods, including oxidative degradated, sulfated and carboxymethylated modification, were conducted and nine MFPs derivatives were obtained. The molecular structures of MFPs were studied and the results showed that MFP-1, MFP-2 and MFP-3, mainly composed of Ara, Gal, Rha, Glc and GalA, with a molecular weight approximately of 20.93 kDa, 56.23 kDa and 118.76 kDa, respectively. FT-IR spectrum of these MFPs all revealed the characteristic polysaccharide absorption bands, while the MFPs derivatives also exhibited some differences due to the structure modification. Hepatoprotective activities tests showed that MFP derivatives could significantly improve the alcohol dehydrogenase (ADH) activity by comparing with the native MFP. And among them, the C-MFP exhibited higher protection effect than the other two modification derivatives.
在本文中，从梅叶冬青中成功提取了三种纯化的梅叶冬青多糖（MFP-1、MFP-2 和 MFP-3）。通过氧化降解、硫酸化和羧甲基化修饰等结构修饰方法，得到了 9 种 MFPs 衍生物。对 MFPs 的分子结构进行了研究，结果表明，MFP-1、MFP-2 和 MFP-3 主要由 Ara、Gal、Rha、Glc 和 GalA 组成，分子量分别约为 20.93 kDa、56.23 kDa 和 118.76 kDa。这些 MFPs 的 FT-IR 光谱均显示出特征多糖吸收带，而 MFPs 衍生物由于结构修饰也表现出一些差异。肝保护活性测试表明，与天然 MFP 相比，MFP 衍生物可显著提高醇脱氢酶（ADH）活性。其中，C-MFP 比其他两种修饰衍生物表现出更高的保护作用。
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