Shanxi Provincial People's Hospital, Taiyuan, China.
Bharat Institute of Technology, Hyderabad, Telangana, India.
Int J Biol Macromol. 2020 Jun 15;153:533-538. doi: 10.1016/j.ijbiomac.2020.02.313. Epub 2020 Feb 27.
Polysaccharides are extensively used in drug delivery systems due to their ability to undergo a broad range of chemical and enzymatic reactions, forming new molecules. Among these, chitosan (CS) and pectin (PEC) are widely used for designing new conjugates and biopolymers.
In this study, we synthesized pectin-chitosan conjugate (PEC-CS) by using carbodiimide crosslinking chemistry. Pectin-N-hydroxysuccinimide ester, formed in the presence of dicyclohexylcarbodiimide under anhydrous conditions, was conjugated with chitosan by linking the free carboxyl group of PEC with the primary amino group of chitosan.
Fourier-transform infrared spectroscopy confirmed the formation of the PEC-CS conjugate. X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, and scanning electron microscopy analyses showed that the PEC-CS conjugate is amorphous in nature, has high thermostability than those of native polymers, and has no cytotoxicity.
Our results indicated that PEC-CS conjugate can be further developed for use in drug delivery systems.
由于多糖能够进行广泛的化学和酶反应，形成新的分子，因此它们被广泛应用于药物传递系统中。壳聚糖（CS）和果胶（PEC）是广泛用于设计新的缀合物和生物聚合物的多糖。
在这项研究中，我们使用碳二亚胺交联化学合成了果胶-壳聚糖缀合物（PEC-CS）。在无水条件下，二环己基碳二亚胺的存在下形成了果胶-N-羟基琥珀酰亚胺酯，通过将 PEC 的游离羧基与壳聚糖的伯氨基连接，将 PEC-N-羟基琥珀酰亚胺酯与壳聚糖缀合。
傅里叶变换红外光谱证实了 PEC-CS 缀合物的形成。X 射线衍射、差示扫描量热法、热重分析和扫描电子显微镜分析表明，PEC-CS 缀合物本质上是无定形的，比天然聚合物具有更高的热稳定性，并且没有细胞毒性。
我们的结果表明，PEC-CS 缀合物可以进一步开发用于药物传递系统。
