Differential Responses by Human Macrophages to Infection With and Non-tuberculous Mycobacteria.

() and non-tuberculous mycobacteria (NTM) are formidable causes of lung diseases throughout the world. While is considered to be more virulent than NTM, host factors also play a key role in disease development. To elucidate whether there are differential immune responses to various mycobacteria, THP-1 macrophages were temporally infected with H37Rv or with four different NTM species. We found that cells infected with had greater bacterial burden and p65 nuclear factor-kappa B (NF-κB) activation than cells infected with NTM. There was also differential expression of mRNA for interleukin-1-β (IL-1β), IL-8, IL-10, and tumor necrosis factor-alpha (TNF-α) with no distinct pattern of mRNA expression among the different mycobacteria. In contrast, at the protein level, some generalizations can be made of the cytokines and chemokines expressed. Compared to uninfected cells, the rapid-growing but not induced significantly greater pro-inflammatory cytokines and IL-10, whereas both NTM individually induced greater levels of chemokines. Compared to uninfected control cells, the two slow-growing NTM and differentially induced cytokine expression with inducing more pro-inflammatory cytokines and IL-10, whereas , , and inducing greater but similar levels of chemokines. -infected THP-1 cells also demonstrated lower level of phagosome-lysosome fusion and apoptosis than NTM-infected cells while there were differences in these macrophage functions among the NTM species. Interestingly, , and have similar levels of autophagosome formation, but the levels displayed by all three were lower than for and . This study demonstrates the differences in bacterial burden and macrophage effector functions among several clinically relevant mycobacterial species. Such disparities may, in part, account for differences in clinical outcomes among patients infected with various species of NTM as has been seen for different strains of .