Pinto Ricardo J B, Bispo Daniela, Vilela Carla, Botas Alexandre M P, Ferreira Rute A S, Menezes Ana C, Campos Fábio, Oliveira Helena, Abreu Maria H, Santos Sónia A O, Freire Carmen S R
Department of Chemistry, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.
Phantom-G, Department of Physics, CICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, Portugal.
Materials (Basel). 2020 Feb 28;13(5):1076. doi: 10.3390/ma13051076.
Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from , as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line.
金纳米颗粒（AuNPs）是研究最多的纳米系统之一，在生物医学应用（包括癌症治疗）方面具有巨大潜力。尽管已经描述了一些基于金的系统，但使用能够控制其性质的绿色且快速的方法至关重要。因此，本研究报道了一种一分钟微波辅助合成岩藻依聚糖包覆的AuNPs的方法，该纳米颗粒尺寸可控且具有高抗肿瘤活性。这些纳米颗粒是使用从[具体来源未给出]中提取的富含岩藻依聚糖的部分作为还原剂和封端剂合成的。对于岩藻依聚糖浓度分别在0.5%至0.05%（w/v）之间的情况，随后形成的单分散球形纳米颗粒的直径极小，在5.8至13.4纳米之间，并且在不同溶液和培养基中具有出色的胶体稳定性。此外，这些纳米颗粒对三种人类肿瘤细胞系（MNT - 1、HepG2和MG - 63）具有抗肿瘤活性，并且流式细胞术结合暗场成像证实了MG - 63细胞系对纳米颗粒的细胞摄取。
