中国海南分离的烈性 alginolyticus 噬菌体 VAP7 的特性及全基因组序列。
Characteristics and complete genome sequence of the virulent Vibrio alginolyticus phage VAP7, isolated in Hainan, China.
机构信息
Department of Graduate, Hebei North University, Zhangjiakou, 075000, China.
Department of Respiratory and Critical Care Diseases, The Fifth Medical Center, Chinese General Hospital of the PLA, Beijing, 100071, China.
出版信息
Arch Virol. 2020 Apr;165(4):947-953. doi: 10.1007/s00705-020-04535-4. Epub 2020 Mar 4.
A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio phage VP-1. Annotation results identified 193 open reading frames and one transfer RNA-encoding gene in the phage genome. The morphology and the results of phylogenetic analysis suggest that VAP7 should be classified as a new member of the family Ackermannviridae. Moreover, phage VAP7 grew over a wide pH (5.0-10.0) and temperature (4-40 °C) range. Host-range experiments revealed that VAP7 could infect 31 Vibrio alginolyticus strains. Thus, VAP7 infecting Vibrio alginolyticus strains represents a potential new candidate for use in phage therapy.
一种新型的噬菌VAP7 从中国海南省三亚市采集的海水中分离得到。全基因组测序分析表明，噬菌体 VAP7 具有线性、双链 DNA 基因组，大小为 144685bp，平均 GC 含量为 41.9%，与弧菌噬菌体 VP-1 具有高度的序列相似性。注释结果在噬菌体基因组中鉴定出 193 个开放阅读框和一个 tRNA 编码基因。形态学和系统发育分析结果表明，VAP7 应被归类为 Ackermannviridae 科的一个新成员。此外，噬菌体 VAP7 在较宽的 pH（5.0-10.0）和温度（4-40°C）范围内生长。宿主范围实验表明，VAP7 可以感染 31 株 Algino-lyticus 弧菌。因此，感染 Algino-lyticus 弧菌的噬菌体 VAP7 代表了噬菌体治疗的一个潜在的新候选者。
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