Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes 35 Keyan Road, Zhunan 35053, Taiwan.
Int J Mol Sci. 2020 Mar 2;21(5):1699. doi: 10.3390/ijms21051699.
The progress of wound regeneration relies on inflammation management, while neovascular angiogenesis is a critical aspect of wound healing. In this study, the bioactive core and corona synergism of quantized gold (QG) were developed to simultaneously address these complicated issues, combining the abilities to eliminate endotoxins and provide oxygen. The QG was constructed from ultrasmall nanogold and a loosely packed amine-based corona via a simple process, but it could nonetheless eliminate endotoxins (a vital factor in inflammation also called lipopolysaccharides) and provide oxygen in situ for the remodeling of wound sites. Even while capturing endotoxins through electrostatic interactions, the catalytic active sites inside the nanogold could maintain its surface accessibility to automatically transform the overexpressed hydrogen peroxide in hypoxic wound regions into oxygen. Since the inflammatory stage is an essential stage of wound healing, the provision of endotoxin clearance by the outer organic corona of the QG could slow inflammation in a way that subsequently promoted two other important stages of wound bed healing, namely proliferation and remodeling. Relatedly, the efficacy of two forms of the QG, a liquid form and a dressing form, was demonstrated at wound sites in this study, with both forms promoting the development of granulation, including angiogenesis and collagen deposition. Thus, the simply fabricated dual function nanocomposite presented herein not only offers reduced batch-to-batch variation but also increased options for homecare treatments.
创伤再生的进展依赖于炎症管理，而新血管生成是创伤愈合的关键方面。在这项研究中，量化金（QG）的生物活性核心和冠协同作用被开发出来，以同时解决这些复杂的问题，结合消除内毒素和提供氧气的能力。QG 通过简单的过程由超小金纳米颗粒和松散堆积的基于胺的冠组成，但它仍然可以消除内毒素（炎症的重要因素，也称为脂多糖）并原位提供氧气，用于创伤部位的重塑。即使通过静电相互作用捕获内毒素，纳米金内部的催化活性位点也可以保持其表面可及性，从而自动将缺氧创伤区域中过表达的过氧化氢转化为氧气。由于炎症阶段是创伤愈合的重要阶段，QG 的外有机冠提供的内毒素清除可以减缓炎症，从而促进创伤床愈合的另外两个重要阶段，即增殖和重塑。相关地，在这项研究中，在创伤部位证明了两种形式的 QG（液体形式和敷料形式）的功效，这两种形式都促进了肉芽组织的发育，包括血管生成和胶原蛋白沉积。因此，本文提出的简单制造的双重功能纳米复合材料不仅提供了降低批间变化的可能性，而且为家庭护理治疗提供了更多选择。
