Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
Int J Mol Sci. 2020 Mar 2;21(5):1709. doi: 10.3390/ijms21051709.
Manganese oxidizing bacteria can produce biogenic manganese oxides (BMO) on their cell surface and have been applied in the fields of agriculture, bioremediation, and drinking water treatment to remove toxic contaminants based on their remarkable chemical reactivity. Herein, we report for the first time the synthetic application of the manganese oxidizing bacteria, MnB1 as a whole-cell biocatalyst for the effective oxidation of β-keto ester with excellent yield. Differing from known chemical protocols toward this transformation that generally necessitate the use of organic solvents, stoichiometric oxygenating agents and complex chemical catalysts, our strategy can accomplish it simply under aqueous and mild conditions with higher efficiency than that provided by chemical manganese oxides. Moreover, the live MnB1 bacteria are capable of continuous catalysis for this C-O bond forming reaction for several cycles and remain proliferating, highlighting the favorable merits of this novel protocol for sustainable chemistry and green synthesis.
锰氧化菌可以在其细胞表面产生生物成因的锰氧化物（BMO），并基于其显著的化学反应活性，已被应用于农业、生物修复和饮用水处理等领域，以去除有毒污染物。在此，我们首次报道了锰氧化菌 MnB1 的合成应用，它作为一种全细胞生物催化剂，可有效地氧化β-酮酯，产率优异。与通常需要使用有机溶剂、化学计量的氧化剂和复杂化学催化剂的已知化学方案不同，我们的策略可以在水相和温和条件下简单地完成这一转化，效率比化学锰氧化物更高。此外，活的 MnB1 细菌能够在几个循环中持续催化这一 C-O 键形成反应，并且仍在增殖，突出了这一新颖方案在可持续化学和绿色合成方面的优势。
