TU Wien, Institute of Applied Synthetic Chemistry, Getreidemarkt 9, 163-OC, 1060 Vienna, Austria.
Curr Opin Chem Biol. 2019 Apr;49:84-90. doi: 10.1016/j.cbpa.2018.10.016. Epub 2018 Nov 17.
Mimicking Nature by biocatalytic cascade reactions in a whole-cell environment is a revolutionary development in multistep synthesis for the production of bulk and fine chemicals. In the past decade, several proof of concept success stories demonstrated the power of those synthetic cascades and paved the road for future industrial applications. Although enzymes and their promiscuity are best suited to construct such artificial pathways, the complexity and the lack of understanding of the cellular machinery slowed down this progress significantly. In this review, recent achievements in the field of whole-cell biocatalysis are described, challenges and hidden traps that have to be overcome are depicted, and strategies are illustrated how to increase overall cascade productivity.
在全细胞环境中通过生物催化级联反应模拟自然是多步合成生产大宗化学品和精细化学品的革命性发展。在过去的十年中，有几个概念验证成功案例证明了这些合成级联的强大，并为未来的工业应用铺平了道路。尽管酶及其多功能性最适合构建此类人工途径，但细胞机制的复杂性和缺乏理解严重阻碍了这一进展。在这篇综述中，描述了全细胞生物催化领域的最新成就，描绘了必须克服的挑战和潜在的陷阱，并说明了如何提高整体级联生产力的策略。
