Cancer Biology Department, Biochemistry Unit, National Cancer Institute, Cairo University, Cairo, Egypt.
Faculty of Pharmacy, Pharmacognosy Department, Ain Shams University, Cairo, Egypt.
Nat Prod Res. 2021 Nov;35(21):4133-4138. doi: 10.1080/14786419.2020.1736064. Epub 2020 Mar 5.
Metabolic profiling of the hexane extract and essential oil of Lam., an economically important agroforestry species, was performed using GC-FID and GC-MS. Cytotoxicity was assessed in MCF-7 cancer cells using sulphorhodamine-B assay. Caspase-8, BcL-2 and Ki-67 levels were evaluated using enzyme-linked immunosorbent assay. Forty-one compounds were identified in the hexane extract, meanwhile, eighty compounds were annotated in the essential oil. n-Decane (9.52%), β-sitosterol (8.13%), undecane (6.32%) and palmitaldehyde (5.50 %) were the major compounds identified in the hexane extract, whereas, β-caryophyllene (32.0%), germacrene D (10.12%) and δ-cadinene (7.20%) predominated in the essential oil. The half maximal inhibitory concentrations (IC) of the extract and oil were 4.55 µg/mL and 12.90 µg/mL, respectively. Caspase-8 activity increased significantly in hexane extract treated cells. Furthermore, BcL-2 and Ki-67 protein expression levels were significantly decreased. Lam exhibit potent cytotoxicity against breast cancer cell line, most likely through apoptosis regulation.
采用 GC-FID 和 GC-MS 对经济上重要的农林树种黄皮的正己烷提取物和精油进行代谢物分析。采用磺基罗丹明 B assay 评估 MCF-7 癌细胞的细胞毒性。通过酶联免疫吸附试验评估 caspase-8、BcL-2 和 Ki-67 水平。在正己烷提取物中鉴定出 41 种化合物，而在精油中注释了 80 种化合物。在正己烷提取物中，主要的化合物为正癸烷（9.52%）、β-谷甾醇（8.13%）、十一烷（6.32%）和棕榈醛（5.50%），而在精油中β-石竹烯（32.0%）、大根香叶烯 D（10.12%）和δ-杜松烯（7.20%）占主导地位。提取物和油的半最大抑制浓度（IC）分别为 4.55 µg/mL 和 12.90 µg/mL。正己烷提取物处理的细胞中 caspase-8 活性显著增加。此外，BcL-2 和 Ki-67 蛋白表达水平显著降低。黄皮对乳腺癌细胞系具有较强的细胞毒性，可能通过凋亡调节。
