Böhm U, Merétey K, Seszták M, Hodinka L, Koó E, Zahumenszky Z
Landesinstitut für Rheumatologie und Physiotherapie, Budapest, Ungarn.
Z Rheumatol. 1988 May-Jun;47(3):161-5.
The opsonizing capacity of sera from 22 patients with rheumatoid arthritis (RA), from 14 with psoriatic arthritis (AP), from seven with ankylosing spondylitis, and from healthy control persons was investigated by luminol-dependent chemiluminescence, induced during yeast phagocytosis of normal polymorphonuclear leukocytes. The chemiluminescence response using opsonizing sera was compared to that induced by no-opsonized yeast and the opsonizing capacity was expressed as a percentage. For the opsonization, fresh native serum, in some experiments Mg2+-EGTA and EDTA-treated serum, was used. In RA and AP sera, a significantly diminished opsonizing capacity (p less than 0.005) was observed. In healthy controls and in SPA patients, the opsonizing capacity of their sera was over 200%, while in seronegative RA patients, it was only 175%, in seropositive RA 125%, and in AP 150% was measured. There was no correlation between opsonizing capacity and complement or immunoglobulin content of the investigated sera. The amount of C3b, IgG and IgM covalently bound to yeast particles was determined, too. Yeast particles bind significantly less (p less than 0.01) IgG when opsonized with RA and AP sera, while a higher relative amount of IgM (p less than 0.01) was bound to yeast incubated in the sera of seropositive RA patients. No significant differences in the C3b binding were observed.
通过鲁米诺依赖的化学发光法研究了22例类风湿性关节炎（RA）患者、14例银屑病关节炎（AP）患者、7例强直性脊柱炎患者以及健康对照者血清的调理吞噬能力，该化学发光由正常多形核白细胞吞噬酵母过程中诱导产生。将使用调理血清时的化学发光反应与未调理酵母诱导的反应进行比较，调理能力以百分比表示。调理时使用新鲜天然血清，在某些实验中使用Mg2+-EGTA和EDTA处理的血清。在RA和AP血清中，观察到调理能力显著降低（p<0.005）。在健康对照者和SPA患者中，其血清的调理能力超过200%，而在血清阴性的RA患者中，仅为175%，血清阳性的RA患者中为125%，AP患者中为150%。所研究血清的调理能力与补体或免疫球蛋白含量之间无相关性。还测定了共价结合到酵母颗粒上的C3b、IgG和IgM的量。当用RA和AP血清调理时，酵母颗粒结合的IgG显著减少（p<0.01），而在血清阳性RA患者血清中孵育的酵母结合的IgM相对量较高（p<0.01）。未观察到C3b结合的显著差异。
