Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, China.
Ecotoxicol Environ Saf. 2022 May 1;236:113482. doi: 10.1016/j.ecoenv.2022.113482. Epub 2022 Mar 31.
The lacewing, Chrysoperla sinica, is a predaceous insect that is important in crop pest management. Chemical pesticides have adversely impacted predaceous insect species. Here we studied the effect of tolfenpyrad on C. sinica. The acute toxicity of tolfenpyrad to the second-instar larvae was determined and indicated that tolfenpyrad is a medium-risk insecticide. Sublethal concentrations (LC, LC, and LC) of tolfenpyrad had effects on the development, reproduction, and predatory ability of C. sinica. When the second-instar larvae of C. sinica were exposed to sublethal concentrations of tolfenpyrad, the activities of protective enzymes, such as superoxide dismutase, peroxidase, and catalase, and detoxification enzymes, including carboxylesterase, glutathione-S-transferase, and P450 monooxygenases, were increased with exposure time. The second-instar larvae of C. sinica exposed to sublethal concentrations of tolfenpyrad exhibited an oxidative stress response that increased the levels of malondialdehyde and reactive oxygen species (ROS). Within 48-120 h after treatment, the contents of mitochondrial respiratory chain complex I and adenosine triphosphate in the second-instar larvae were decreased. This resulted in an imbalance between the production and clearance of ROS and caused cellular damage.
草蛉，又名中华通草蛉，是一种捕食性昆虫，在作物害虫管理中起着重要作用。化学农药对捕食性昆虫物种产生了不利影响。在这里，我们研究了氟啶虫胺腈对中华通草蛉的影响。测定了氟啶虫胺腈对二龄幼虫的急性毒性，表明氟啶虫胺腈是一种中风险杀虫剂。亚致死浓度（LC、LC 和 LC）的氟啶虫胺腈对中华通草蛉的发育、繁殖和捕食能力有影响。当中华通草蛉的二龄幼虫暴露于亚致死浓度的氟啶虫胺腈时，保护酶（如超氧化物歧化酶、过氧化物酶和过氧化氢酶）和解毒酶（包括羧酸酯酶、谷胱甘肽-S-转移酶和 P450 单加氧酶）的活性随着暴露时间的增加而增加。暴露于亚致死浓度氟啶虫胺腈的中华通草蛉二龄幼虫表现出氧化应激反应，增加了丙二醛和活性氧（ROS）的水平。在处理后 48-120 小时内，二龄幼虫中线粒体呼吸链复合物 I 和三磷酸腺苷的含量降低。这导致 ROS 的产生和清除之间的失衡，并导致细胞损伤。
