Moon H W, Nagy B, Isaacson R E, Orskov I
Infect Immun. 1977 Feb;15(2):614-20. doi: 10.1128/iai.15.2.614-620.1977.
Several strains of enterotoxigenic Escherichia coli (ETEC) isolated from pigs were found to have an antigen (K99) previously reported only on strains of calf and lamb origin and which facilitates intestinal colonization in the latter two species. Several human ETEC were also tested for K99; however, none were positive. Each of four K99-positive ETEC strains of calf origin and one of pig origin produced K99 in pig ileum in vivo, adhered to villous epithelium in pig ileum, colonized pig ileum, and caused profuse diarrhea in newborn pigs. In contrast to the K99-positive strains above, four K99-negative ETEC from humans and chickens and one K99-positive ETEC from a calf either did not colonize pig ileum or did so inconsistently. When the K99-negative strains did colonize, they had little or no tendency to adhere to intestinal villi. These results are consistent with the hypothesis that K99 facilitates adhesion to and colonization of pig ileum by some ETEC.
从猪身上分离出的几种产肠毒素大肠杆菌（ETEC）菌株被发现有一种抗原（K99），该抗原此前仅在源自小牛和羔羊的菌株上被报道过，并且它有助于后两种动物在肠道内定殖。对几株人源ETEC也进行了K99检测；然而，结果均为阴性。四株源自小牛的K99阳性ETEC菌株和一株源自猪的K99阳性ETEC菌株在猪回肠内均能在体内产生K99，附着于猪回肠的绒毛上皮，定殖于猪回肠，并导致新生仔猪出现大量腹泻。与上述K99阳性菌株不同，四株源自人和鸡的K99阴性ETEC以及一株源自小牛的K99阳性ETEC要么无法在猪回肠内定殖，要么定殖情况不稳定。当K99阴性菌株确实定殖时，它们几乎没有或根本没有附着于肠绒毛的倾向。这些结果与K99有助于某些ETEC附着于猪回肠并在其中定殖的假说一致。
