Departamento de Protección de Cultivos, Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), 14004 Córdoba, Spain.
Viruses. 2020 Mar 3;12(3):279. doi: 10.3390/v12030279.
A novel mycovirus named Fusarium oxysporum f. sp. dianthi mitovirus 1 (FodMV1) has been identified infecting a strain of f. sp. from Colombia. The genome of FodMV1 is 2313 nt long, and comprises a 172-nt 5'-UTR, a 2025-nt single ORF encoding an RdRp of 675 amino acid residues, and a 113-nt 3´-UTR. Homology BlastX searches identifies FodMV1 as a novel member of the genus in the family . As the rest of mitoviruses, the genome of FodMV1 presents a high percentage of A+U (58.8%) and contains a number of UGA codons that encode the amino acid tryptophan rather than acting as stop codons as in the universal genetic code. Another common feature with other mitoviruses is that the 5'- and 3'-UTR regions of FodMV1 can be folded into potentially stable stem-loop structures. Result from phylogenetic analysis place FodMV1 in a different clade than the rest of mitoviruses described in other spp. Incidence of FodMV1-infections in the collection of f. sp. isolates analyzed is relatively high. Of particular interest is the fact that FodMV1 has been detected infecting isolates from two geographical areas as distant as Spain and Colombia.
一种名为尖孢镰刀菌百合专化型线叶病毒 1（FodMV1）的新型真菌病毒已被鉴定感染来自哥伦比亚的尖孢镰刀菌百合专化型菌株。FodMV1 的基因组长 2313 个核苷酸，包含一个 172 个核苷酸的 5'-UTR、一个编码 675 个氨基酸残基的 RdRp 的 2025 个核苷酸的单一 ORF，以及一个 113 个核苷酸的 3'-UTR。BlastX 同源性搜索将 FodMV1 鉴定为 家族 中的一个新型属成员。与其他线叶病毒一样，FodMV1 的基因组具有较高的 A+U 百分比（58.8%），并且包含许多 UGA 密码子，这些密码子编码色氨酸而不是像通用遗传密码那样作为终止密码子。与其他线叶病毒的另一个共同特征是，FodMV1 的 5'-和 3'-UTR 区域可以折叠成潜在稳定的茎环结构。系统发育分析的结果将 FodMV1 置于与其他描述的尖孢镰刀菌属不同的分支中。在所分析的尖孢镰刀菌百合专化型分离物中，FodMV1 感染的发生率相对较高。特别有趣的是，FodMV1 已被检测到感染来自西班牙和哥伦比亚等两个遥远地理区域的分离物。
