Department of Dental Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Biology Oral, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
BMC Oral Health. 2020 Mar 6;20(1):65. doi: 10.1186/s12903-020-1049-1.
High concentrations of Co, Ni, and Cr in the blood serum of dental technicians are strongly associated with free radical formation. It has highly reactive properties that can cause further oxidation of molecule in the vicinity.
This study intended to investigate whether the Dental Technician occupational exposure of Co, Ni and Cr, could contribute to the high incidence of cancer.
This was a cross-sectional study to dental technicians, performed after acccepting ethical clearance. Blood was sampled in 3 examinations for Co, Ni, Cr using Atomic Absorbance Spectrophotometry (AAS), MDA was examined with TBARS test, also 8 OHdG and wildtype p53 proteins determined by ELISA method.
Comparative statistical analysis, showing a significant difference (p < 0.05) between levels of Co, Ni, and Cr in exposed groups to the control group. But, not all variables was proven to be positively correlated, only with Cr, and Co, and negatively correlated with wild-type p53.
MDA,8-OHdG and wildtype p53 can be used as genotoxic biomarkers in the metal exposed group, since they can accurately reflect the degree of Oxidative damage.
血清中钴、镍和铬浓度较高的牙科技术人员与自由基的形成密切相关。它具有高度反应性，可以导致附近分子的进一步氧化。
本研究旨在探讨牙科技术人员职业性接触钴、镍和铬是否会导致癌症发病率升高。
这是一项横断面研究，对牙科技术人员进行了三次 Co、Ni、Cr 血样采集，采用原子吸收光谱法（AAS）检测，TBARS 法检测 MDA，ELISA 法检测 8-OHdG 和野生型 p53 蛋白。
统计学比较分析显示，暴露组与对照组之间 Co、Ni 和 Cr 水平存在显著差异（p<0.05）。但并非所有变量都呈正相关，仅与 Cr 和 Co 呈正相关，与野生型 p53 呈负相关。
MDA、8-OHdG 和野生型 p53 可作为金属暴露组的遗传毒性生物标志物，因为它们可以准确反映氧化损伤程度。
