Genome-wide identification and characterization of the lateral organ boundaries domain gene family in var.

The genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various species. However, members of the gene family have yet to be identified in var. In the present study, fifty-nine genes were identified and distributed on 10 chromosomes. The BrrLBD proteins are predicted to encode hydrophobic polypeptides between 118 and 394 amino acids in length and with molecular weights ranging from 13.31 to 44.24 kDa; the theoretical pI for these proteins varies from 4.83 to 9.68. There were 17 paralogous gene pairs in the BrrLBD family, suggesting that the amplification of the gene family involved large-scale gene duplication events. Members of the BrrLBD family were divided into 7 subclades (class I a to e, class II a and b). Analysis of gene structure and conserved domains revealed that most genes of the same subclade had similar gene structures and protein motifs. The expression profiles of 59 genes were determined through Quantitative Real-time fluorescent PCR (qRT-PCR). Most genes in the same subclade had similar gene expression profiles. However, the expression patterns of 7 genes differed from their duplicates, indicating that although the gene function of most BrrLBD genes has been conserved, some BrrLBD genes may have undergone evolutionary change.