Yu Qin, Hu Simin, Du Jiancan, Yang Yongping, Sun Xudong
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China.
Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Plant Divers. 2019 Dec 19;42(1):52-60. doi: 10.1016/j.pld.2019.11.004. eCollection 2020 Feb.
The genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various species. However, members of the gene family have yet to be identified in var. In the present study, fifty-nine genes were identified and distributed on 10 chromosomes. The BrrLBD proteins are predicted to encode hydrophobic polypeptides between 118 and 394 amino acids in length and with molecular weights ranging from 13.31 to 44.24 kDa; the theoretical pI for these proteins varies from 4.83 to 9.68. There were 17 paralogous gene pairs in the BrrLBD family, suggesting that the amplification of the gene family involved large-scale gene duplication events. Members of the BrrLBD family were divided into 7 subclades (class I a to e, class II a and b). Analysis of gene structure and conserved domains revealed that most genes of the same subclade had similar gene structures and protein motifs. The expression profiles of 59 genes were determined through Quantitative Real-time fluorescent PCR (qRT-PCR). Most genes in the same subclade had similar gene expression profiles. However, the expression patterns of 7 genes differed from their duplicates, indicating that although the gene function of most BrrLBD genes has been conserved, some BrrLBD genes may have undergone evolutionary change.
这些基因编码高度保守的植物特异性LOB结构域蛋白，可调节多种物种的生长和发育。然而，在该变种中尚未鉴定出该基因家族的成员。在本研究中，鉴定出59个该基因并分布在10条染色体上。预测BrrLBD蛋白编码长度在118至394个氨基酸之间、分子量在13.31至44.24 kDa之间的疏水性多肽；这些蛋白的理论pI在4.83至9.68之间变化。BrrLBD家族中有17对旁系同源基因对，表明该基因家族的扩增涉及大规模基因复制事件。BrrLBD家族成员分为7个亚分支（I类a至e，II类a和b）。基因结构和保守结构域分析表明，同一亚分支的大多数该基因具有相似的基因结构和蛋白质基序。通过定量实时荧光PCR（qRT-PCR）测定了59个该基因的表达谱。同一亚分支的大多数该基因具有相似的基因表达谱。然而，7个基因的表达模式与其复制基因不同，表明尽管大多数BrrLBD基因的功能保守，但一些BrrLBD基因可能经历了进化变化。
