Golestan Research Center of Gastroenterology and Hepatology (GRCGH), School of Health, Golestan University of Medical Sciences (GOUMS), Gorgan, Iran.
Digestive Disease Research Center, Digestive Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Turk J Gastroenterol. 2020 Feb;31(2):99-104. doi: 10.5152/tjg.2020.181028.
BACKGROUND/AIMS: The interaction of CD40 ligand (CD40L) and CD40 triggers the induction of pro-inflammatory cytokines. It has been proposed that vitamin D deficiency might be an important factor, which causes or aggregates the autoimmune situations. The aim of the present study was to assess the effect of vitamin D on CD40L gene expression in patients with ulcerative colitis (UC).
Ninety mild-to-moderate UC patients were randomized to receive a single injection of 7.5 mg cholecalciferol or 1 mL normal saline. At baseline and 90 days following the intervention, RNA samples from whole blood were obtained. Fold changes in CD40L mRNA expression were determined for each patient using the 2-ΔΔCq method. The data were analyzed.
The serum levels of vitamin D and calcium increased only in the vitamin D group (p<0.05). Relative to baseline values, the CD40L gene expression fold change was significantly lower in the vitamin D group compared with the placebo group (median±interquartile range: 0.34±0.30 vs 0.43±1.20, respectively, p=0.016).
The results of this study showed that vitamin D administration in mild-to-moderate UC patients led to the downregulation of the CD40L gene, which is an essential part of inflammatory pathways.
背景/目的：CD40 配体（CD40L）与 CD40 的相互作用触发了促炎细胞因子的诱导。有人提出，维生素 D 缺乏可能是一个重要的因素，它导致或聚集自身免疫情况。本研究的目的是评估维生素 D 对溃疡性结肠炎（UC）患者 CD40L 基因表达的影响。
90 例轻中度 UC 患者被随机分为接受单次 7.5mg 胆钙化醇或 1ml 生理盐水注射。在干预前和 90 天后，从全血中获取 RNA 样本。使用 2-ΔΔCq 法确定每位患者 CD40L mRNA 表达的倍数变化。对数据进行分析。
只有在维生素 D 组中，血清维生素 D 和钙水平增加（p<0.05）。与基线值相比，维生素 D 组的 CD40L 基因表达倍数变化明显低于安慰剂组（中位数±四分位距：0.34±0.30 与 0.43±1.20，分别为 p=0.016）。
本研究结果表明，在轻中度 UC 患者中给予维生素 D 治疗可导致 CD40L 基因下调，这是炎症途径的重要组成部分。
