Sengul Aysen Taslak, Buyukkkarabacak Yasemin Bilgin, Altunkaynak Berrin Zuhal, Yetim Tulin Durgun, Altun Gamze Yayla, Sengul Bilal, Basoglu Ahmet
a Department of Thoracic Surgery , Ondokuz Mayis University , Samsun , Turkey.
b Department of Histology and Embryology , Ondokuz Mayis University , Turkey.
Acta Chir Belg. 2017 Feb;117(1):21-28. doi: 10.1080/00015458.2016.1210874. Epub 2016 Aug 3.
In cases of congenital chest wall deformities, it is important to maintain the flexibility of the chest wall after rib cartilage resection. In this study, we aimed to determine the regeneration capability of cartilage and the effects of platelet-rich plasma (PRP) on the regeneration process.
A total of 16 four-week-old New Zealand rabbits were used in this study. In the 4th-5th right costal cartilages, the perichondrial sheaths were dissected and costal cartilages were excised. Then, the perichondrial sheaths were closed with absorbable material in the sham group (n = 8), and this was done after replacing PRP in the PRP group (n = 8). The left costal cartilages of the animals were used as controls. The volumes of the costal cartilages and their perichondrial sheaths were estimated using Cavalieri's principle. In addition, the mean numerical densities of the chondroblasts and chondrocytes per square millimetre were estimated using unbiased counting frames.
In the PRP and sham groups, the volumes of the cartilages and perichondrial sheaths were higher than those of the control group (p < 0.05). The numerical densities of the chondroblasts and chondrocytes increased more in the PRP group than in the sham group (p < 0.05).
Applying PRP after resection may provide better healing and faster regeneration of cartilage.
在先天性胸壁畸形病例中，肋骨软骨切除术后保持胸壁的柔韧性很重要。在本研究中，我们旨在确定软骨的再生能力以及富血小板血浆（PRP）对再生过程的影响。
本研究共使用了16只4周龄的新西兰兔。在右侧第4 - 5肋软骨处，切开软骨膜鞘并切除肋软骨。然后，假手术组（n = 8）用可吸收材料封闭软骨膜鞘，PRP组（n = 8）则在注入PRP后进行封闭。动物的左侧肋软骨用作对照。使用卡瓦列里原理估算肋软骨及其软骨膜鞘的体积。此外，使用无偏计数框估算每平方毫米内成软骨细胞和软骨细胞的平均数值密度。
PRP组和假手术组中，软骨和软骨膜鞘的体积均高于对照组（p < 0.05）。PRP组中成软骨细胞和软骨细胞的数值密度增加幅度大于假手术组（p < 0.05）。
切除术后应用PRP可能会使软骨愈合更好且再生更快。
