从红树林内生真菌 Phomopsis sp. SYSUQYP-23 中分离得到的生物碱的抗炎活性。

Anti-inflammatory activities of alkaloids from the mangrove endophytic fungus Phomopsis sp. SYSUQYP-23.

机构信息

School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, PR China; School of Marin Sciences, Sun Yat-sen University, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510006, PR China.

School of Pharmacy, Guangdong Medical University, Dongguan 523808, PR China.

出版信息

Bioorg Chem. 2020 Apr;97:103712. doi: 10.1016/j.bioorg.2020.103712. Epub 2020 Feb 28.

DOI:10.1016/j.bioorg.2020.103712

PMID:32146180

Abstract

Five new maleimide derivatives, (+)- and (-)- farinomalein F (1), (+)- and (-)- farinomalein G (2), farinomalein H (3) and one new linearly fused prenylated indole alkaloid phomoamide (8), along with five known compounds 4-7 and 9 were isolated from the mangrove endophytic fungus Phomopsis sp. SYSUQYP-23. Their structures and absolute configurations were determined by HRESIMS, spectroscopic and electronic circular dichroism (ECD) calculations. Bioassay results showed that compounds 3-9 exhibited significant inhibitory activities against nitric oxide (NO) production in lipopolysaccharides (LPS) induced RAW 264.7 cells, with IC values ranging from 4.5 to 25 μM. Moreover, the molecular docking study implied the probable binding interaction of compounds 4 and 5 with nitric oxide synthase.

摘要

从红树林内生真菌 Phomopsis sp. SYSUQYP-23 中分离得到五个新的马来酰亚胺衍生物 (+)- 和 (-)-farinomalein F (1)、(+)- 和 (-)-farinomalein G (2)、farinomalein H (3)，以及一个新的线性稠合的prenylated 吲哚生物碱 phomoamide (8)，此外还分离得到五个已知化合物 4-7 和 9。通过高分辨质谱、光谱和电子圆二色性（ECD）计算确定了它们的结构和绝对构型。生物活性测试结果表明，化合物 3-9 对脂多糖（LPS）诱导的 RAW 264.7 细胞中一氧化氮（NO）的产生具有显著的抑制活性，IC 值范围为 4.5-25 μM。此外，分子对接研究表明化合物 4 和 5 可能与一氧化氮合酶具有结合相互作用。