Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, People's Republic of China.
Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong Universty of Science and Technology, Wuhan 430070, Hubei Province, People's Republic of China.
Fitoterapia. 2021 Apr;150:104840. doi: 10.1016/j.fitote.2021.104840. Epub 2021 Jan 31.
Five new compounds, including a pair of diphenylcyclopentenone enantiomers (±)-phomopsisin A (1), a sesquiterpenoid 15-hydroxylithocarin A (2), a new diketopiperazine alkaloid prenylcyclotryprostatin A (3) and 7-hydroxy-cis-L(-)-3,6-dibenzyl-2,5-dioxopiperazine (6), along with five known compounds were isolated from the fungus Phomopsis asparagi. Their structures were elucidated on the basis of spectroscopic analyses (1D and 2D NMR), theoretical electronic circular dichroism (ECD) calculation, modified Mosher's method, and X-ray crystallography. The racemates of (±)-phomopsisin A showed inhibition on α-glucosidase with IC of 30.07 ± 0.75 μM (positive control acarbose, 121 ± 2.7 μM).
从真菌芦笋拟盘多毛孢中分离得到五个新化合物，包括一对二苯环戊烯酮对映体（±）-phomopsisin A（1）、一种倍半萜 15-羟基金刚烷 A（2）、一种新的二酮哌嗪生物碱prenylcyclotryprostatin A（3）和 7-羟基顺式-L(-)-3,6-二苄基-2,5-二氧代哌嗪（6），以及五个已知化合物。根据光谱分析（1D 和 2D NMR）、理论电子圆二色性（ECD）计算、改良的 Mosher 法和 X 射线晶体学，确定了它们的结构。（±）-phomopsisin A 的外消旋体对 α-葡萄糖苷酶具有抑制作用，IC 为 30.07±0.75μM（阳性对照阿卡波糖，121±2.7μM）。
