Medical Physiology Department, Faculty of Medicine, Minia University, Minia, 61111, Egypt.
Histology and Cell Biology Department, Minia University, Faculty of Medicine, Minia, 61111, Egypt.
J Trace Elem Med Biol. 2020 Jul;60:126480. doi: 10.1016/j.jtemb.2020.126480. Epub 2020 Feb 27.
Acute pancreatitis is a serious condition with multi-factorial etiology. The negative impact of acute pancreatitis on the exocrine pancreatic function is well documented; however, its impact on the endocrine function needs more elucidation. Our study aimed to investigate the effect of Nano-Selenium (Nano-Se) on both pancreatic functions in acute pancreatitis.
l-arginine induced acute pancreatitis in rats was used as a model. Fifty adult male albino rats were separated into groups: 1- control group (C), 2- C+ Nano-Se, 3-acute pancreatitis group (AP) and 4- AP+ Nano-Se. Nano-Se was administered before induction of acute pancreatitis. Serum levels of amylase, lipase, selenium, glucose, insulin and interleukin-1β (IL-1β) were measured. Homeostatic model assessment of beta cell function (HOMA-β) was also calculated. Oxidative stress markers, selenium content and the anti-apoptotic factor, B-cell leukemia/lymphoma-2 (Bcl-2) were assayed in pancreatic tissue along with immuno-expression of nuclear transcription factor-kappa B (NF-κB).
Acute pancreatitis negatively affected both pancreatic functions. Nano-Se administration lessened the developed pancreatic injury and improved both pancreatic functions.
Nano-Se could improve the deteriorated pancreatic functions in acute pancreatitis via its anti-inflammatory, antioxidant and pro-apoptotic actions. Thus, it may be used in prevention of acute pancreatitis and the associated hyperglycemia in vulnerable individuals such as patients undergoing endoscopic retrograde cholangio-pancreatography.
急性胰腺炎是一种病因复杂的严重疾病。急性胰腺炎对外分泌功能的负面影响已有充分的文献记载；然而，其对内分泌功能的影响仍需进一步阐明。本研究旨在探讨纳米硒（Nano-Se）对急性胰腺炎时胰腺内外分泌功能的影响。
采用精氨酸诱导的急性胰腺炎大鼠模型。将 50 只成年雄性白化大鼠分为 4 组：1-对照组（C）、2-C+Nano-Se、3-急性胰腺炎组（AP）和 4-AP+Nano-Se。在诱导急性胰腺炎前给予 Nano-Se。检测血清淀粉酶、脂肪酶、硒、葡萄糖、胰岛素和白细胞介素-1β（IL-1β）水平。还计算了胰岛β细胞功能的稳态模型评估（HOMA-β）。测定胰腺组织的氧化应激标志物、硒含量和抗凋亡因子 B 细胞淋巴瘤/白血病-2（Bcl-2），以及核转录因子-κB（NF-κB）的免疫表达。
急性胰腺炎对内外分泌功能均有不良影响。Nano-Se 给药减轻了已发展的胰腺损伤并改善了内外分泌功能。
Nano-Se 可能通过抗炎、抗氧化和促凋亡作用改善急性胰腺炎时恶化的胰腺功能。因此，它可能用于预防易发生急性胰腺炎和相关高血糖症的个体，如接受内镜逆行胰胆管造影术的患者。
