The State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science, Fudan University, Shanghai, 200433, China.
Institute of Electrochemical Energy Storage, Helmholtz-Zentrum Berlin für Materialien und Energie, 14109, Berlin, Germany.
Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9617-9623. doi: 10.1002/anie.202000771. Epub 2020 Apr 1.
A protein Pascal triangle has been constructed as new type of supramolecular architecture by using the inducing ligand strategy that we previously developed for protein assemblies. Although mathematical studies on this famous geometry have a long history, no work on such Pascal triangles fabricated from native proteins has been reported so far due to their structural complexity. In this work, by carefully tuning the specific interactions between the native protein building block WGA and the inducing ligand R-SL, a 2D Pascal-triangle lattice with three types of triangular voids has been assembled. Moreover, a 3D crystal structure was obtained based on the 2D Pascal triangles. The distinctive carbohydrate binding sites of WGA and the intralayer as well as interlayer dimerization of RhB was the key to facilitate nanofabrication in solution. This strategy may be applied to prepare and explore various sophisticated assemblies based on native proteins.
通过使用我们之前开发的用于蛋白质组装的诱导配体策略，构建了一种蛋白质帕斯卡三角形，作为新型超分子结构。尽管这种著名的几何结构的数学研究有着悠久的历史，但由于其结构的复杂性，目前还没有报道过由天然蛋白质制成的这种帕斯卡三角形。在这项工作中，通过仔细调整天然蛋白质构建块 WGA 与诱导配体 R-SL 之间的特定相互作用，组装了具有三种三角形空隙的 2D 帕斯卡三角形晶格。此外，还基于 2D 帕斯卡三角形获得了 3D 晶体结构。WGA 的独特碳水化合物结合位点以及 RhB 的层内和层间二聚化是促进溶液中纳米制造的关键。该策略可用于制备和探索基于天然蛋白质的各种复杂组装体。
