Department of Anesthesiology, Wuhan University, Renmin Hospital, Wuhan, 430060 Hubei Province, China.
Mediators Inflamm. 2020 Feb 14;2020:5741047. doi: 10.1155/2020/5741047. eCollection 2020.
Multiple interleukin (IL) family members were reported to be closely related to hypertension. We aimed to investigate whether IL-9 affects angiotensin II- (Ang II-) induced hypertension in mice.
Mice were treated with Ang II, and IL-9 expression was determined. In addition, effects of IL-9 knockout (KO) on blood pressure were observed in Ang II-infused mice. To determine whether the effects of IL-9 on blood pressure was mediated by the signal transducer and activator of the transcription 3 (STAT3) pathway, Ang II-treated mice were given S31-201. Furthermore, circulating IL-9 levels in patients with hypertension were measured.
Ang II treatment increased serum and aortic IL-9 expression in a dose-dependent manner; IL-9 levels were the highest in the second week and continued to remain high into the fourth week after the treatment. IL-9 KO downregulated proinflammatory cytokine expression, whereas it upregulated anti-inflammatory cytokine levels, relieved vascular dysfunction, and decreased blood pressure in Ang II-infused mice. IL-9 also reduced smooth muscle 22 (SM22 (SM22.
IL-9 KO alleviates inflammatory response, prevents phenotypic transformation of smooth muscle, reduces vascular dysfunction, and lowers blood pressure via the STAT3 pathway in Ang II-infused mice. IL-9 might be a novel target for the treatment and prevention of clinical hypertension.
多种白细胞介素（IL）家族成员与高血压密切相关。我们旨在研究白细胞介素 9（IL-9）是否影响小鼠的血管紧张素 II（Ang II）诱导的高血压。
用 Ang II 处理小鼠，并测定 IL-9 的表达。此外，在 Ang II 输注小鼠中观察 IL-9 敲除（KO）对血压的影响。为了确定 IL-9 对血压的影响是否通过信号转导和转录激活因子 3（STAT3）途径介导，用 S31-201 处理 Ang II 处理的小鼠。此外，测量高血压患者的循环 IL-9 水平。
Ang II 处理以剂量依赖性方式增加血清和主动脉中的 IL-9 表达；IL-9 水平在治疗后第二周最高，并持续到第四周。IL-9 KO 下调促炎细胞因子的表达，而上调抗炎细胞因子水平，缓解血管功能障碍，并降低 Ang II 输注小鼠的血压。IL-9 还减少了平滑肌 22（SM22）的表达。
IL-9 KO 通过 Ang II 输注小鼠中的 STAT3 途径减轻炎症反应，防止平滑肌表型转化，减少血管功能障碍，并降低血压。IL-9 可能是治疗和预防临床高血压的新靶点。
