Bouhlali Eimad Dine Tariq, Hmidani Abdelbasset, Bourkhis Bouchra, Khouya Tarik, Ramchoun Mhamed, Filali-Zegzouti Younes, Alem Chakib
National Institute of Agronomic Research Regional Center of Errachidia, Morocco.
Biochemistry of Natural Products Team, Faculty of Sciences and Techniques Errachidia, Morocco.
Heliyon. 2020 Feb 19;6(2):e03436. doi: 10.1016/j.heliyon.2020.e03436. eCollection 2020 Feb.
Date ( L.) seeds are seen as good drug to cure rheumatoid arthritis and asthma in Moroccan traditional medicine. The present research aimed to study the anti-inflammatory effect, of methanol extract of different date seed varieties using membrane stabilizing effect, nitric oxide radical scavenging activity, inhibition of protein denaturation, carrageenan-induced paw edema and croton oil induced ear edema. The polyphenolic profile was examined using HPLC-DAD. Rutin, quercetin, -coumaric and caffeic acids were the main among the analysed phenolic compounds. Concerning the anti-inflammatory activity, the analysed date seed were significantly effective in scavenging nitric oxide free radical, in stabilisation of erythrocyte membrane and possessed a high anti denaturation effect. In agreement with this finding, date seed exhibited a profound ability to reduce paw and ear swelling induced by carrageenan and croton oil respectively. The biochemical parameters showed that date seed are able to reduce the erythrocyte sedimentation rate (ERS) and C-reactive protein (CRP) concentration in rats used in Carrageenan-induced paw edema model. The predominant phenolic compounds are the potential candidates that drive these activities and the differences observed among varieties are related to their chemical composition. These data suggest that date seeds can be explored as a therapeutic agent for the treatment of inflammatory diseases.
在摩洛哥传统医学中，椰枣（L.）种子被视为治疗类风湿性关节炎和哮喘的良药。本研究旨在利用膜稳定作用、一氧化氮自由基清除活性、蛋白质变性抑制、角叉菜胶诱导的爪肿胀和巴豆油诱导的耳肿胀，研究不同椰枣种子品种甲醇提取物的抗炎作用。使用高效液相色谱-二极管阵列检测器（HPLC-DAD）检测多酚谱。芦丁、槲皮素、对香豆酸和咖啡酸是分析的酚类化合物中的主要成分。关于抗炎活性，分析的椰枣种子在清除一氧化氮自由基、稳定红细胞膜方面具有显著效果，并具有较高的抗变性作用。与此发现一致，椰枣种子分别表现出显著降低角叉菜胶和巴豆油诱导的爪和耳肿胀的能力。生化参数表明，在角叉菜胶诱导的爪肿胀模型中使用的大鼠中，椰枣种子能够降低红细胞沉降率（ERS）和C反应蛋白（CRP）浓度。主要的酚类化合物是驱动这些活性的潜在候选物，品种间观察到的差异与其化学成分有关。这些数据表明，椰枣种子可作为治疗炎症性疾病的治疗剂进行探索。
