Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, 21045-900, Brazil.
Departamento de Saneamento e Saúde Ambiental, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, 21045-900, Brazil.
Food Environ Virol. 2020 Jun;12(2):130-136. doi: 10.1007/s12560-020-09423-3. Epub 2020 Mar 9.
The availability of drinking water is one of the main determinants of quality of life, disease prevention and the promotion of health. Viruses are important agents of waterborne diseases and have been described as important markers of human faecal contamination. This study aimed to investigate viruses' presence as an indicator of drinking water quality in low-income communities in the Manguinhos area, Rio de Janeiro, Brazil. Three hundred and four drinking water samples (2L/each) were collected along the drinking water distribution-to-consumption pathway in households, as well as healthcare and school units. Water samples were collected both directly from the water supply prior to distribution and after storage in tanks and filtration units. Using qPCR, viruses were detected 50 times in 45 water samples (15%), 19 of these being human adenovirus, 17 rotavirus A and 14 norovirus GII. Viral loads recovered ranged from 5E+10 to 8.7E+10 genome copies/Liter. Co-detection was observed in five household water samples and there was no difference regarding virus detection across sampling sites. Precarious and inadequate environmental conditions characterized by the lack of local infrastructure regarding basic sanitation and waste collection in the territory, as well as negligent hygiene habits, could explain viral detection in drinking water in regions with a water supply system.
饮用水的供应是生活质量、疾病预防和促进健康的主要决定因素之一。病毒是重要的水传播疾病病原体，被认为是人类粪便污染的重要标志物。本研究旨在调查巴西里约热内卢曼久inhos 地区低收入社区饮用水中病毒作为水质指标的存在情况。从家庭、医疗和学校单位的饮用水分配到消费途径中采集了 304 个饮用水样本（每个样本 2L）。在分配前和储存在水箱和过滤装置后直接从供水系统中采集水样。使用 qPCR 方法，在 45 个水样（15%）中检测到 50 次病毒，其中 19 次是人腺病毒，17 次为轮状病毒 A，14 次为诺如病毒 GII。回收的病毒载量范围为 5E+10 至 8.7E+10 基因组拷贝/升。在五个家庭水样中观察到共同检测，并且在不同采样点之间病毒检测没有差异。该地区供水系统中饮用水中检测到病毒，这可能是由于当地基础设施不完善，缺乏基本卫生和废物收集，以及卫生习惯疏忽等不稳定和不充分的环境条件造成的。
