Jahan Sarwat, Fatima Andleeb, Alam Iftikhar, Ullah Asad, Rehman Humaira, Afsar Tayyaba, Almajwal Ali, Razak Suhail
1Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
2Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
BMC Nutr. 2018 Dec 13;4:41. doi: 10.1186/s40795-018-0250-y. eCollection 2018.
CDC's (Centers for Disease Control and Prevention) National Center for Health statistics recent reports have shown that an upsurge has occurred in the use of dietary supplements among age of 20 years since 1994 and this use shown regular increase. The purpose of our study was to investigate the effect of supplements on the reproductive health on male athletes in Pakistan.
A total of 150 adult male with mean age of 25.78 ± 0.56 years were included in this study and divided into four groups: Non-athlete control ( = 57), Non supplemental athlete control ( = 40), Supplemental athlete group I ( = 28) and supplemental athlete group II ( = 25). Blood (10 ml) was taken from each subject. Complete blood count was performed and 5 ml of blood was centrifuged to separate plasma and then analyzed for antioxidant enzyme (CAT, POD, GR and GSH) activities, Lipid peroxidation (TBARS), electrolyte, metal (sodium, potassium and zinc) and Luteinizing hormone (LH) concentration.
Complete blood count results showed normal RBC, WBC, Platelets, Hemoglobin, Hematocrit, Mean corpuscular hemoglobin and Mean corpuscular hemoglobin concentration. Antioxidant enzymes (CAT, POD, GR, GSH) increased significantly in supplemental athletes as compared to control groups. Sodium and potassium showed significant increase ( < 0.001) in supplemental athlete group I, while TBARS also showed significant increase ( < 0.05) in supplemental group I and II as compared to non athlete control while non supplemental athletes showed significant increase ( < 0.05) in TBARS concentration as compared to non athlete control. LH concentration was found to be decreased significantly ( < 0.05) in supplemental group I and II as compared to control groups.
It is therefore concluded from the present results that oxidative stress was considerably elevated in response to supplement consumption among athletes which may affect their health haematological parameters and reproductive hormones.
美国疾病控制与预防中心（CDC）国家卫生统计中心最近的报告显示，自1994年以来，20岁及以上人群使用膳食补充剂的情况呈上升趋势，且这种使用呈持续增长态势。我们研究的目的是调查补充剂对巴基斯坦男性运动员生殖健康的影响。
本研究共纳入150名成年男性，平均年龄为25.78±0.56岁，分为四组：非运动员对照组（n = 57）、不补充膳食补充剂的运动员对照组（n = 40）、补充膳食补充剂的运动员组I（n = 28）和补充膳食补充剂的运动员组II（n = 25）。从每个受试者采集10毫升血液。进行全血细胞计数，并将5毫升血液离心以分离血浆，然后分析抗氧化酶（CAT、POD、GR和GSH）活性、脂质过氧化（TBARS）、电解质、金属（钠、钾和锌）以及促黄体生成素（LH）浓度。
全血细胞计数结果显示红细胞、白细胞、血小板、血红蛋白、血细胞比容、平均红细胞血红蛋白和平均红细胞血红蛋白浓度均正常。与对照组相比，补充膳食补充剂的运动员的抗氧化酶（CAT、POD、GR、GSH）显著增加。补充膳食补充剂的运动员组I中的钠和钾显著增加（P < 0.001），而与非运动员对照组相比，补充膳食补充剂的运动员组I和组II中的TBARS也显著增加（P < 0.05），且与非运动员对照组相比，不补充膳食补充剂的运动员的TBARS浓度也显著增加（P < 0.05）。与对照组相比，补充膳食补充剂的运动员组I和组II中的LH浓度显著降低（P < 0.05）。
因此，从目前的结果可以得出结论，运动员服用补充剂会使氧化应激显著升高，这可能会影响他们的血液学参数和生殖激素。
