Unterberger Sandra, Maier-Salamon Alexandra, Jäger Walter, Wessner Barbara, Wagner Karl-Heinz
Research Platform Active Ageing, University of Vienna, Austria.
Department of Clinical Pharmacy and Diagnostics, University of Vienna, Austria.
Data Brief. 2020 Feb 20;29:105309. doi: 10.1016/j.dib.2020.105309. eCollection 2020 Apr.
Bioavailability of nutrients is highly diverse and depends on a variety of endogenous and exogenous factors in humans. This data article reports on the plasma response of 10 human subjects (5 females, 5 males) to a single dose of a multivitamin drink within 6h (blood taken after 1, 2, 4, and 6h). Nutrients, which were considered in the assessment, were folate (Radioimmuno Assay), vitamin B12 (Radioimmuno Assay) and resveratrol and its plasma metabolites resveratrol-3--glucuronide (R3G), resveratrol-4'--glucuronide (R4G), resveratrol-3--sulfate (R3S) and resveratrol-3--4'--disulfate (RD, all HPLC). Biological outcome measures were malondialdehyde (MDA, HPLC) and Ferric Reducing ability potential (FRAP, Microplate reader). Mean plasma concentration increased over time significantly for folate (p < 0.05, maximum concentration (Tmax) after 2h), R3G, R4G, R3S (all p < 0.05, Tmax after 1h), RD (p < 0.05, Tmax after 2h) as well as MDA, which decreased (p < 0.05, Tmax after 2h). No significant change was observed for vitamin B12 and FRAP. Within this mean development, individual changes of participants were highly diverse such as for folate from +42 to +422%, for MDA from -49 to +30% or vitamin B12 from -4 to +33%. For R4G 4 out of 10 subjects showed even no increase in plasma at all. For R4G plasma response ranged from 0 to 36 ng/ml, for R3G from 0 to 53 ng/ml or for R4S from 62 to 265 ng/ml. There was no gender difference regarding the plasma response.
营养素的生物利用度差异很大，取决于人体多种内源性和外源性因素。本数据文章报告了10名人类受试者（5名女性，5名男性）在6小时内对单剂量多种维生素饮料的血浆反应（在1、2、4和6小时后采血）。评估中考虑的营养素包括叶酸（放射免疫测定法）、维生素B12（放射免疫测定法）以及白藜芦醇及其血浆代谢物白藜芦醇 - 3 - β - 葡萄糖醛酸苷（R3G）、白藜芦醇 - 4' - β - 葡萄糖醛酸苷（R4G）、白藜芦醇 - 3 - β - 硫酸盐（R3S）和白藜芦醇 - 3 - β - 4' - 二硫酸盐（RD，均采用高效液相色谱法）。生物学结果指标为丙二醛（MDA，高效液相色谱法）和铁还原能力（FRAP，酶标仪）。叶酸（p < 0.05，2小时后达到最大浓度（Tmax））、R3G、R4G、R3S（均p < 0.05，1小时后达到Tmax）、RD（p < 0.05，2小时后达到Tmax）以及MDA（p < 0.05，2小时后达到Tmax）的平均血浆浓度随时间显著升高，其中MDA呈下降趋势。维生素B12和FRAP未观察到显著变化。在这种平均变化过程中，参与者的个体变化差异很大，例如叶酸的变化范围为 + 42%至 + 422%，MDA的变化范围为 - 49%至 + 30%，维生素B12的变化范围为 - 4%至 + 33%。对于R4G，10名受试者中有4名血浆根本没有增加。R4G的血浆反应范围为0至36 ng/ml，R3G为0至53 ng/ml，R4S为62至265 ng/ml。血浆反应不存在性别差异。
