Zhu Keming, Zhang Weiwei, Sarwa Rehman, Xu Shuo, Li Kaixia, Yang Yanhua, Li Yulong, Wang Zheng, Cao Jun, Li Yaoming, Tan Xiaoli
Jiangsu University, Institute of Life Sciences, Zhenjiang, Jiangsu, China.
Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, China.
Genet Mol Biol. 2020 Mar 6;43(1):e20190305. doi: 10.1590/1678-4685-GMB-2019-0305. eCollection 2020.
Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence meristem development, but also suggest bioengineering strategies for the improved breeding and production of Brassica napus.
油菜是中国重要的油料作物之一。更好地了解油菜主要农艺性状的调控网络可以提高油菜产量。在本研究中,我们获得了一个花序突变体,其表现出融合表型,类似于拟南芥的clavata样表型,因此我们将该突变体命名为Bnclavata样(Bnclv样)。表型分析表明,花序分生组织(IM)的异常发育导致了融合花序表型。在蛋白质丰度阶段,该突变体中参与代谢过程、活性氧代谢和细胞骨架形成的主要调节因子发生了改变。这些结果不仅揭示了生物学过程与花序分生组织发育之间的关系,还为甘蓝型油菜的改良育种和生产提出了生物工程策略。