Owor Richard Oriko, Bedane Kibrom Gebreheiwot, Zühlke Sebastian, Derese Solomon, Ong'amo George Otieno, Ndakala Albert, Spiteller Michael
Department of Chemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.
Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Strasse 6, 44221 Dortmund, Germany.
J Nat Prod. 2020 Apr 24;83(4):996-1004. doi: 10.1021/acs.jnatprod.9b00922. Epub 2020 Mar 10.
Phytochemical analysis of a methanol-dichloromethane (1:1) extract of the aerial parts of led to the isolation of 18 compounds. Seven of these, namely, lineaflavones A-D (-), 6-methoxygeraldone (), 8″-acetylobovatin (), and 5-hydroxy-7-methoxysaniculamin A () are new compounds. The compounds were characterized based on their NMR and HRMS data. The anti-inflammatory effects of the crude extract and isolated compounds were evaluated by measuring the levels of interleukins (IL-1β, IL-2, and IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). The crude extract inhibited the release of all cytokines except IL-1β, which slightly increased in comparison to the LPS control. All the tested compounds suppressed the production of IL-2, GM-CSF, and TNF-α. Whereas compounds , , -, -, , and decreased production of IL-6, compounds , , , , , -, and inhibited the release of IL-1β. It is worth noting that most of the compounds tested showed a superior reduction in cytokines release compared to the reference drug ibuprofen.
对某植物地上部分的甲醇 - 二氯甲烷（1:1）提取物进行植物化学分析，从中分离出18种化合物。其中7种，即线黄酮A - D（-）、6 - 甲氧基杰拉尔多酮（）、8″ - 乙酰欧波瓦亭（）和5 - 羟基 - 7 - 甲氧基沙尼库拉明A（）是新化合物。这些化合物通过其核磁共振（NMR）和高分辨质谱（HRMS）数据进行表征。通过测量脂多糖（LPS）刺激的外周血单核细胞（PBMCs）中白细胞介素（IL - 1β、IL - 2和IL - 6）、粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）和肿瘤坏死因子 - α（TNF - α）的水平，评估粗提物和分离出的化合物的抗炎作用。粗提物抑制了除IL - 1β之外的所有细胞因子的释放，与LPS对照组相比，IL - 1β略有增加。所有测试化合物均抑制IL - 2、GM - CSF和TNF - α的产生。化合物、、 - 、 - 、和降低了IL - 6的产生，而化合物、、、、、 - 和抑制了IL - 1β的释放。值得注意的是，与参比药物布洛芬相比，大多数测试化合物在细胞因子释放减少方面表现更优。
