Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.
Department of Chemistry, Higher Teacher Training College, University of Yaounde I, P.O. Box 47, Yaounde, Cameroon.
Sci Rep. 2024 Aug 6;14(1):18211. doi: 10.1038/s41598-024-68364-0.
Sorindeia nitidula (Anacardiaceae) is used by traditional practitioners to treat influenza illnesses with cephalgia and febrile aches. However, the potential active ingredients for its remarkable antioxidant, anti-HIV and antitrypanosomal activities remain unexplored. The present study aims to evaluate the antioxidant, anti-HIV and antitrypanosomal activities of the ethyl acetate extract of S. nitidula (SN) in order to screen out the bioactive compounds and to analyze their possible mechanisms of action. Overall, 21 phenolic compounds were annotated, by using the MS and MS/MS information provided by the QTOF-MS. In vitro assays on the extract revealed potent antioxidant (IC = 0.0129 ± 0.0001 mg/mL), anti-HIV (IC = 1.736 ± 0.036 µM), antitrypanosomal (IC = 1.040 ± 0.010 µM) activities. Furthermore, SN did not present cytotoxic effect on HeLa cancer cell lines. The integrated strategy based on LC-ESI-QTOF-MS/MS provided a powerful tool and a multidimensional perspective for further exploration of active ingredients in S. nitidula responsible for the antioxidant, anti-HIV and antitrypanosomal activities.
光亮千瓣木（漆树科）被传统医生用于治疗伴有头痛和发热的流感疾病。然而，其具有显著抗氧化、抗 HIV 和抗锥虫活性的潜在活性成分仍未被探索。本研究旨在评估光亮千瓣木的乙酸乙酯提取物（SN）的抗氧化、抗 HIV 和抗锥虫活性，以筛选出生物活性化合物并分析其可能的作用机制。总的来说，通过 QTOF-MS 提供的 MS 和 MS/MS 信息，注释了 21 种酚类化合物。体外实验表明，该提取物具有很强的抗氧化活性（IC=0.0129±0.0001 mg/mL）、抗 HIV 活性（IC=1.736±0.036 µM）和抗锥虫活性（IC=1.040±0.010 µM）。此外，SN 对 HeLa 癌细胞系没有细胞毒性作用。基于 LC-ESI-QTOF-MS/MS 的综合策略为进一步探索光亮千瓣木中负责抗氧化、抗 HIV 和抗锥虫活性的活性成分提供了一种强大的工具和多维视角。
