评估暴露于伏马菌素 B1（FB1）期间小鼠体内离子动态平衡失衡和细胞色素 P450 系统紊乱。

Assessment of ionic homeostasis imbalance and cytochrome P450 system disturbance in mice during fumonisin B1 (FB1) exposure.

机构信息

College of Life Science and Engineering, Foshan University, Foshan, Guangdong, 528231, PR China.

出版信息

Chemosphere. 2020 Jul;251:126393. doi: 10.1016/j.chemosphere.2020.126393. Epub 2020 Mar 2.

DOI:10.1016/j.chemosphere.2020.126393

Abstract

Fumonisin B1 (FB1) is a mycotoxin frequently found in agricultural commodities, and poses a considerable risk for human and animal health. The aim of this study was to investigate the toxic effect of FB1 in mice intestine. Male Kunming mice (n = 40) were treated with FB1 diet for 42 days. Histopathological and biochemical analyses, including ion concentrations, transcription of ATPase subunits and mRNA expression of cytochrome P450s (CYP450s) analyses were performed on duodenum, cecum and colon of mice. The results revealed that FB1 caused histological alterations, including partial shedding of villous epithelial cells and inflammatory cell infiltration. Furthermore, a significant change in Na, K and Ca in serum, and the mRNA expression of ATPase subunits and CYP450s in intestinal tracts were observed in FB1-exposed mice. Our results suggested that FB1 exposure induce histopathological injury via disrupting CYP isoforms transcription and triggering ion homeostasis imbalance in mice intestinal tracts.

摘要

伏马菌素 B1（FB1）是一种经常在农产品中发现的真菌毒素，对人类和动物的健康构成了相当大的风险。本研究旨在研究 FB1 在小鼠肠道中的毒性作用。雄性昆明小鼠（n=40）用 FB1 饮食处理 42 天。对小鼠的十二指肠、盲肠和结肠进行组织病理学和生化分析，包括离子浓度、ATP 酶亚基转录和细胞色素 P450s（CYP450s）的 mRNA 表达分析。结果表明，FB1 导致组织学改变，包括绒毛上皮细胞部分脱落和炎症细胞浸润。此外，在 FB1 暴露的小鼠中，血清中 Na、K 和 Ca 以及肠道中 ATP 酶亚基和 CYP450s 的 mRNA 表达发生了显著变化。我们的结果表明，FB1 暴露通过破坏 CYP 同工酶的转录和触发小鼠肠道中的离子平衡失调来诱导组织病理学损伤。