Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
These authors contributed equally to this work.
Euro Surveill. 2020 Mar;25(9). doi: 10.2807/1560-7917.ES.2020.25.9.2000173.
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
当德国面临欧洲首例与旅行相关的严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 暴发时，及时建立诊断检测的需求就产生了。我们描述了在一次大规模接触者追踪调查中的实验室经验，比较了不同 PCR 系统中先前发表的实时 RT-PCR 检测方法和一种商业试剂盒。我们发现，使用不同 PCR 系统的相同引物和探针的检测方法性能存在差异，而商业试剂盒的性能良好。
