Ozatik Fikriye Yasemin, Ozatik Orhan, Yigitaslan Semra, Kaygısız Bilgin, Erol Kevser
Department of Pharmacology, Kutahya Health Sciences University School of Medicine, Kutahya, Turkey.
Department of Histology and Embriology, Kutahya Health Sciences University School of Medicine, Kutahya, Turkey.
Eurasian J Med. 2020 Feb;52(1):6-11. doi: 10.5152/eurasianjmed.2019.19044.
In this study, the aim is to observe changes induced by dehydroepiandrosterone (DHEA) and resveratrol (RES) in diminished ovarian follicles that was induced by 4-vinylcyclohexenediepoxide (VCD).
Twenty four Wistar albino female rats were divided into 3 groups: control, DHEA and RES. Unilateral oophorectomy was performed in control group to remove the right ovary of 4 rats and the left ovary of 4 rats. After administration of 160 mg/kg VCD, remaining ovaries were removed. Following the same VCD treatment, in DHEA and RES groups, 60 mg/kg DHEA and 20 mg/kg RES were given for 45 days respectively and residual ovaries were removed. Hematoxylin-eosin and TUNEL staining were performed. Follicle stimulating hormone (FSH), estradiol (E2) and anti-mullerian hormone (AMH) values were measured.
In control group, VCD-induced apoptosis in follicles increased the TUNEL-positive cell counts (p<0.001) with decreased number of follicles. On the other hand, DHEA significantly increased all three follicle types in the ovaries and decreased apoptosis (p<0.001). The decreased follicle number in all three follicle types after VCD treatment were found to be significantly increased after RES treatment (p<0.001). Apoptosis in the follicles was significantly decreased by RES administration (p<0.001). FSH values were found to be increased with VCD and to reach control values with DHEA and RES. E2 values significantly decreased with VCD, but significantly increased with RES and DHEA.
Both DHEA and RES may improve VCD-induced diminished ovarian reserve. DHEA and RES increased the number of primary, primordial and growing follicles, with no significant difference between them.
本研究旨在观察脱氢表雄酮（DHEA）和白藜芦醇（RES）对由4-乙烯基环己烯二环氧物（VCD）诱导的卵巢卵泡减少所引起变化的影响。
将24只Wistar白化雌性大鼠分为3组：对照组、DHEA组和RES组。对照组进行单侧卵巢切除术，切除4只大鼠的右侧卵巢和4只大鼠的左侧卵巢。给予160mg/kg VCD后，切除剩余卵巢。在相同的VCD处理后，DHEA组和RES组分别给予60mg/kg DHEA和20mg/kg RES，持续45天，然后切除残余卵巢。进行苏木精-伊红染色和TUNEL染色。测量卵泡刺激素（FSH）、雌二醇（E2）和抗苗勒管激素（AMH）的值。
在对照组中，VCD诱导的卵泡凋亡增加了TUNEL阳性细胞计数（p<0.001），同时卵泡数量减少。另一方面，DHEA显著增加了卵巢中所有三种卵泡类型的数量，并减少了细胞凋亡（p<0.001）。RES处理后，发现VCD处理后所有三种卵泡类型中减少的卵泡数量显著增加（p<0.001）。RES给药显著降低了卵泡中的细胞凋亡（p<0.001）。发现FSH值随VCD升高，而DHEA和RES使其达到对照值。E2值随VCD显著降低，但随RES和DHEA显著升高。
DHEA和RES均可改善VCD诱导的卵巢储备减少。DHEA和RES增加了初级卵泡、原始卵泡和生长卵泡的数量，两者之间无显著差异。
