T Mortezazadeh, E Gholibegloo, N Riyahi Alam, S Haghgoo, A E Musa, M Khoobi
PhD, Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran.
PhD, Department of Medical Physics, School of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
J Biomed Phys Eng. 2020 Feb 1;10(1):25-38. doi: 10.31661/jbpe.v0i0.1018. eCollection 2020 Feb.
Glucose transporter (Glut), a cellular transmembrane receptor, has a key role in the metabolism of cell glucose and is also associated with various human carcinomas.
In this study, we evaluated a magnetic resonance (MR) imaging contrast agent for tumor detection based on paramagnetic gadolinium oxide (GdO) coated polycyclodextrin (PCD) and modified with glucose (GdO@PCD-Glu) for the targeting of overexpressed glucose receptors.
In this experimental study, 3T magnetic resonance imaging (MRI) scanner was used to assess the specific interactions between Glut1-overexpressing tumor cells (MDA-MB-231) and GdO@PCD-Glu NPs. Furthermore, the capacity of transporting GdO@PCD-Glu NPs to tumor cells was evaluated.
It was found that the acquired MRI T signal intensity of MDA-MB-231 cells that were treated with the GdO@PCD-Glu NPs increased significantly. Based on the results obtained, GdO@PCD-Glu NPs can be applied in targeting Glut1-overexpressing tumor cells in vivo, as well as an MRI-targeted tumor agent to enhance tumor diagnosis.
Results have shown that glucose-shell of magnetic nanoparticles has a key role in diagnosing cancer cells of high metabolic activity.
葡萄糖转运蛋白（Glut）是一种细胞跨膜受体，在细胞葡萄糖代谢中起关键作用，且与多种人类癌症相关。
在本研究中，我们评估了一种基于顺磁性氧化钆（GdO）包覆的聚环糊精（PCD）并经葡萄糖修饰（GdO@PCD-Glu）的磁共振（MR）成像造影剂，用于靶向过表达的葡萄糖受体以检测肿瘤。
在本实验研究中，使用3T磁共振成像（MRI）扫描仪评估过表达Glut1的肿瘤细胞（MDA-MB-231）与GdO@PCD-Glu纳米粒子之间的特异性相互作用。此外，评估了GdO@PCD-Glu纳米粒子向肿瘤细胞转运的能力。
发现用GdO@PCD-Glu纳米粒子处理的MDA-MB-231细胞所获得的MRI T信号强度显著增加。基于所得结果，GdO@PCD-Glu纳米粒子可用于体内靶向过表达Glut1的肿瘤细胞，以及作为MRI靶向肿瘤剂以增强肿瘤诊断。
结果表明磁性纳米粒子的葡萄糖壳层在诊断高代谢活性癌细胞中起关键作用。
