de Siqueira-Santos R, Sardella-Silva G, Nascimento M A, Teixeira de Oliveira L, Coelho-Sampaio T, Ribeiro-Resende V T
Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Neuroquímica, Centro de Ciências da Saúde Bl. C, Cidade Universitária, 21949-900 Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica Leopoldo de Meis, Laboratório de Agregação de Proteínas e Amiloidoses, Centro de Ciências da Saúde, Bl. E, Cidade Universitária, 21949-900 Rio de Janeiro, RJ, Brazil.
Mater Today Bio. 2019 Aug 21;3:100026. doi: 10.1016/j.mtbio.2019.100026. eCollection 2019 Jun.
Unlike the central nervous system, peripheral nerves can regenerate after injury. However, depending on the size of the lesion, the endogenous regenerative potential is not enough to replace the lost nerve tissue. Many strategies have been used to generate biomaterials capable of restoring nerve functions. Here, we set out to investigate whether adsorbing the extracellular matrix protein, laminin (LM), to poly-ℇ-caprolactone (PCL) filaments would enhance functional nerve regeneration. Initial studies showed that explants of dorsal root ganglia (DRGs) of P1 neonate mice exhibited stronger neuritogenesis on a substrate of LM that had been previously polymerized (polylaminin [polyLM]) than on ordinary LM. On the other hand, when silicone tubes filled with PCL filaments were used to bridge a 10-mm sciatic nerve gap in rats, only filaments coated with LM improved tissue replacement beyond that obtained with empty tubes. Motor function recovery correlated with tissue replacement as only LM-coated filaments consistently improved motor skills. Finally, analysis of the lateral gastrocnemius muscle revealed that the LM group presented twice the amount of α-bungarotixin-labeled motor plates. In conclusion, although polyLM was more effective in stimulating growth of sensory fibers out of DRGs , LM adsorbed to PCL filaments exhibited the best regenerative properties in inducing functional motor recovery after peripheral injury .
与中枢神经系统不同，周围神经在损伤后能够再生。然而，根据损伤的大小，内源性再生潜力不足以替代丢失的神经组织。人们已经采用了许多策略来制备能够恢复神经功能的生物材料。在此，我们着手研究将细胞外基质蛋白层粘连蛋白（LM）吸附到聚ε-己内酯（PCL）细丝上是否会增强功能性神经再生。初步研究表明，新生P1小鼠背根神经节（DRG）外植体在先前聚合的LM（聚层粘连蛋白[polyLM]）基质上比在普通LM上表现出更强的神经突生长。另一方面，当使用填充有PCL细丝的硅胶管桥接大鼠10毫米的坐骨神经间隙时，只有涂有LM的细丝比空管更能促进组织替代。运动功能恢复与组织替代相关，因为只有涂有LM的细丝持续改善了运动技能。最后，对腓肠外侧肌的分析表明，LM组的α-银环蛇毒素标记的运动终板数量是对照组的两倍。总之，尽管polyLM在刺激DRG中的感觉纤维生长方面更有效，但吸附到PCL细丝上的LM在诱导周围神经损伤后的功能性运动恢复方面表现出最佳的再生特性。
