Department of Public Health, School of Medicine, Dokkyo Medical University.
Office of Epidemiology, Radiation Medical Science Center for the Fukushima Health Management Survey, Fukushima Medical University.
J Atheroscler Thromb. 2020 Oct 1;27(10):1086-1096. doi: 10.5551/jat.51664. Epub 2020 Mar 11.
The association between urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative stress marker, and the incidence of cardiovascular disease (CVD) has not been confirmed because no previous studies evaluated 24-hour 8-OHdG excretion levels in the general population. We aimed to confirm the association between 24-hour urinary 8-OHdG levels and CVD risk among Japanese men and women.
A nested case-control study was performed based on a 24-hour urine collection in a community-based cohort study performed from 1996 to 2005. Seventy-six cases (55 men and 21 women) who experienced their first CVD incidence during the follow-up period (median: 5.9 years) were recruited. The controls were frequency-matched 1:2, with each case for sex, age, area of residence, and baseline year. The 8-OHdG level was measured by enzyme-linked immunosorbent assay. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using conditional logistic regression models adjusted for body mass index, ethanol intake, smoking status, and estimated glomerular filtration rate.
The geometric mean and geometric standard deviation (SD) of 8-OHdG levels (nmol/day) for cases and controls were 35.5 (1.55) and 35.5 (1.54) for men and 32.1 (1.35) and 25.0 (1.39) for women, respectively. The multivariable OR (95% CI) of CVD incidence according to the 1-SD increment of the log-transformed 8-OHdG level was 2.08 (0.99-4.37) for women. The multivariable ORs (95% CIs) for the 1st (lowest) and 4th versus 2nd quartile according to 8-OHdG for men were 3.29 (1.02-10.61) and 2.77 (0.96-7.96), respectively.
A high 8-OHdG level tended to be associated with CVD incidence among women.
由于先前的研究并未评估过一般人群的 24 小时尿 8-羟基-2'-脱氧鸟苷（8-OHdG）排泄水平，因此，氧化应激标志物尿 8-羟基-2'-脱氧鸟苷（8-OHdG）与心血管疾病（CVD）的发病风险之间的关联尚未得到证实。本研究旨在证实日本男性和女性的 24 小时尿 8-OHdG 水平与 CVD 风险之间的关联。
本研究基于 1996 年至 2005 年进行的一项以社区为基础的队列研究中的 24 小时尿液收集，开展了一项巢式病例对照研究。在随访期间（中位随访时间：5.9 年），共纳入 76 例（55 名男性和 21 名女性）首次发生 CVD 的病例。根据性别、年龄、居住地区和基线年份，以 1:2 的频率匹配对照。通过酶联免疫吸附试验测量 8-OHdG 水平。采用条件 logistic 回归模型，在调整了体重指数、乙醇摄入量、吸烟状况和估算肾小球滤过率后，计算比值比（OR）和 95%置信区间（CI）。
病例组和对照组的 8-OHdG 水平（nmol/天）的几何均数和几何标准差（SD）分别为男性 35.5（1.55）和 35.5（1.54），女性 32.1（1.35）和 25.0（1.39）。按对数转换后 8-OHdG 水平每增加 1SD，女性 CVD 发病风险的多变量 OR（95%CI）为 2.08（0.99-4.37）。按 8-OHdG 四分位距将男性分为第 1 （最低） quartile 与第 4 quartile 与第 2 quartile 时，多变量 OR（95%CI）分别为 3.29（1.02-10.61）和 2.77（0.96-7.96）。
8-OHdG 水平较高与女性 CVD 发病风险之间存在一定关联。
