CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
University of Chinese Academy of Sciences, Beijing, China.
Nat Prod Res. 2021 Nov;35(21):3810-3819. doi: 10.1080/14786419.2020.1739046. Epub 2020 Mar 12.
One strain many compounds (OSMAC) strategy was an effective method to activate the silent biosynthetic genes of microorganisms. Comparison with our previous investigations on the secondary metabolites of the marine-derived fungus sp. SCSIO 41501, in this study, three new cyclopentenone derivatives, aspergispones A-C (), and five new cyclohexenone derivatives, aspergispones D-H (), together with two known analogues, were further isolated from the fungal strain by altering culture medium compositions. The structures of new compounds were elucidated by extensive spectroscopic analysis. And the absolute configurations of and were further confirmed by single-crystal X-ray diffraction experiments.
一种菌株多种化合物（OSMAC）策略是激活微生物沉默生物合成基因的有效方法。与我们之前对海洋来源真菌 SCSIO 41501 的次生代谢产物的研究相比，在本研究中，通过改变培养基组成，从真菌菌株中进一步分离出三种新的环戊烯酮衍生物aspergispones A-C()，以及五种新的环己烯酮衍生物 aspergispones D-H()，同时还分离得到了两种已知类似物。新化合物的结构通过广泛的光谱分析得到阐明。并且通过单晶 X 射线衍射实验进一步确定了和的绝对构型。
